Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells

Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells

Advanced glycation end products (AGEs) excessive accumulation in the body either by exogenous intake or endogenous formation makes individuals prone to oxidative stress and inflammation damage. In this study, DCFH-DA fluorescence test and qPCR experiment were carried out to study the effect of some...

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Main Authors: Jing Teng, Jun Li, Yueliang Zhao, Mingfu Wang
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:Journal of Functional Foods
Subjects:
Hesperetin
Advanced glycation endproducts
Oxidative stress
Inflammation
RNA-seq
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464621001298
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spelling doaj-e04489cb30604ccb8578d5dcf2bd08912021-05-12T04:10:08ZengElsevierJournal of Functional Foods1756-46462021-06-0181104480Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cellsJing Teng0Jun Li1Yueliang Zhao2Mingfu Wang3College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China; College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR ChinaDepartment of Infectious Diseases and Public Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China; Corresponding authors at: School of Biological Sciences, The University of Hong Kong, Hong Kong, China (M. Wang).College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China; School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Corresponding authors at: School of Biological Sciences, The University of Hong Kong, Hong Kong, China (M. Wang).Advanced glycation end products (AGEs) excessive accumulation in the body either by exogenous intake or endogenous formation makes individuals prone to oxidative stress and inflammation damage. In this study, DCFH-DA fluorescence test and qPCR experiment were carried out to study the effect of some dietary flavonoids on AGEs induced toxicity. Among four tested flavonoids, it was found that hesperetin could significantly inhibit both AGEs-induced ROS production and gene expressions of TNF-α, IL-1β, IL-6, MCP-1, COX-2 and iNOS. High-throughput transcriptome analysis showed that the protective effect of hesperetin against AGEs-induced toxicity might be attributed to inhibit the activation of MAPK, JAK, NF-κB-related pathways. Furthermore, gene set enrichment analysis was performed using the MSigDB database and twenty-three key genes related were screened. This study might provide new ideas for the prevention of AGEs-related diseases and lay a theoretical foundation for the application of dietary flavonoids.http://www.sciencedirect.com/science/article/pii/S1756464621001298HesperetinAdvanced glycation endproductsOxidative stressInflammationRNA-seq
collection DOAJ
language English
format Article
sources DOAJ
author Jing Teng
Jun Li
Yueliang Zhao
Mingfu Wang
spellingShingle Jing Teng
Jun Li
Yueliang Zhao
Mingfu Wang
Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells
Journal of Functional Foods
Hesperetin
Advanced glycation endproducts
Oxidative stress
Inflammation
RNA-seq
author_facet Jing Teng
Jun Li
Yueliang Zhao
Mingfu Wang
author_sort Jing Teng
title Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells
title_short Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells
title_full Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells
title_fullStr Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells
title_full_unstemmed Hesperetin, a dietary flavonoid, inhibits AGEs-induced oxidative stress and inflammation in RAW264.7 cells
title_sort hesperetin, a dietary flavonoid, inhibits ages-induced oxidative stress and inflammation in raw264.7 cells
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2021-06-01
description Advanced glycation end products (AGEs) excessive accumulation in the body either by exogenous intake or endogenous formation makes individuals prone to oxidative stress and inflammation damage. In this study, DCFH-DA fluorescence test and qPCR experiment were carried out to study the effect of some dietary flavonoids on AGEs induced toxicity. Among four tested flavonoids, it was found that hesperetin could significantly inhibit both AGEs-induced ROS production and gene expressions of TNF-α, IL-1β, IL-6, MCP-1, COX-2 and iNOS. High-throughput transcriptome analysis showed that the protective effect of hesperetin against AGEs-induced toxicity might be attributed to inhibit the activation of MAPK, JAK, NF-κB-related pathways. Furthermore, gene set enrichment analysis was performed using the MSigDB database and twenty-three key genes related were screened. This study might provide new ideas for the prevention of AGEs-related diseases and lay a theoretical foundation for the application of dietary flavonoids.
topic Hesperetin
Advanced glycation endproducts
Oxidative stress
Inflammation
RNA-seq
url http://www.sciencedirect.com/science/article/pii/S1756464621001298
work_keys_str_mv AT jingteng hesperetinadietaryflavonoidinhibitsagesinducedoxidativestressandinflammationinraw2647cells
AT junli hesperetinadietaryflavonoidinhibitsagesinducedoxidativestressandinflammationinraw2647cells
AT yueliangzhao hesperetinadietaryflavonoidinhibitsagesinducedoxidativestressandinflammationinraw2647cells
AT mingfuwang hesperetinadietaryflavonoidinhibitsagesinducedoxidativestressandinflammationinraw2647cells
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