Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus

Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus

Myeloid leukemia factor (MLF) plays an important role in the development of Drosophila. It is reported that MLF could inhibit white spot syndrome virus (WSSV) replication in shrimp. However, the function and mechanism of MLF in shrimp antibacterial immunity are still elusive. In this study, we foun...

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Bibliographic Details
Main Authors: X W Feng, J L Huo, X Z Shi
Format: Article
Language:English
Published: University of Modena and Reggio Emilia 2018-06-01
Series:Invertebrate Survival Journal
Subjects:
shrimp
MjMLF
V. anguillarum
AMPs
Mj14-3-3
Online Access:https://isj02.unimore.it/index.php/ISJ/article/view/426
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spelling doaj-e02613ec4ced4f5f8f02a4f1245feb512020-12-02T18:25:40ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-06-01151Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicusX W Feng0J L Huo1X Z Shi2Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China Myeloid leukemia factor (MLF) plays an important role in the development of Drosophila. It is reported that MLF could inhibit white spot syndrome virus (WSSV) replication in shrimp. However, the function and mechanism of MLF in shrimp antibacterial immunity are still elusive. In this study, we found MjMLF could be upregulated by Vibro anguillarum challenge in hemocytes, gills, and intestine of kuruma shrimp. RNAi assay showed that MjMLF could facilitate bacterial clearance in shrimp, and it was beneficial for shrimp survival post V. anguillarum infection. Pull down assay showed that rMjMLF could bind to rMj14-3-3 in vitro. Some antimicrobial peptide genes could be regulated by MjMLF. The results indicated that MjMLF might participate in the anti-bacterial immune reaction of shrimp. https://isj02.unimore.it/index.php/ISJ/article/view/426shrimpMjMLFV. anguillarumAMPsMj14-3-3
collection DOAJ
language English
format Article
sources DOAJ
author X W Feng
J L Huo
X Z Shi
spellingShingle X W Feng
J L Huo
X Z Shi
Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus
Invertebrate Survival Journal
shrimp
MjMLF
V. anguillarum
AMPs
Mj14-3-3
author_facet X W Feng
J L Huo
X Z Shi
author_sort X W Feng
title Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus
title_short Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus
title_full Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus
title_fullStr Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus
title_full_unstemmed Myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp Marsupenaeus japonicus
title_sort myeloid leukemia factor participates in the antibacterial immune reaction of kuruma shrimp marsupenaeus japonicus
publisher University of Modena and Reggio Emilia
series Invertebrate Survival Journal
issn 1824-307X
publishDate 2018-06-01
description Myeloid leukemia factor (MLF) plays an important role in the development of Drosophila. It is reported that MLF could inhibit white spot syndrome virus (WSSV) replication in shrimp. However, the function and mechanism of MLF in shrimp antibacterial immunity are still elusive. In this study, we found MjMLF could be upregulated by Vibro anguillarum challenge in hemocytes, gills, and intestine of kuruma shrimp. RNAi assay showed that MjMLF could facilitate bacterial clearance in shrimp, and it was beneficial for shrimp survival post V. anguillarum infection. Pull down assay showed that rMjMLF could bind to rMj14-3-3 in vitro. Some antimicrobial peptide genes could be regulated by MjMLF. The results indicated that MjMLF might participate in the anti-bacterial immune reaction of shrimp.
topic shrimp
MjMLF
V. anguillarum
AMPs
Mj14-3-3
url https://isj02.unimore.it/index.php/ISJ/article/view/426
work_keys_str_mv AT xwfeng myeloidleukemiafactorparticipatesintheantibacterialimmunereactionofkurumashrimpmarsupenaeusjaponicus
AT jlhuo myeloidleukemiafactorparticipatesintheantibacterialimmunereactionofkurumashrimpmarsupenaeusjaponicus
AT xzshi myeloidleukemiafactorparticipatesintheantibacterialimmunereactionofkurumashrimpmarsupenaeusjaponicus
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