The relation between Escherichia coli surface functional groups' composition and their physicochemical properties Relação entre a composição dos grupos funcionais da superfície de Escherichia coli e suas propriedades fisicoquímicas

Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectros...

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Bibliographic Details
Main Authors: Fatima Hamadi, Hassan Latrache, Hafida Zahir, Abderrahmene Elghmari, Mohamed Timinouni, Mostapha Ellouali
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2008-03-01
Series:Brazilian Journal of Microbiology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100003
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Summary:Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.<br>Características de superfície de Escherichia coli, tais como hidrofobicidade, mobilidade eletroforética e composição dos grupos funcionais de superfície, foram estudadas. Essas características foram determinadas por medidas de angulo de contato com água, microeletroforese e espectroscopia fotoeletrônica de raio-X (XPS), respectivamente. A relação entre as propriedades fisicoquímicas e a composição dos grupos funcionais foi também examinada. Aparentemente, a mobilidade eletroforética em pH 7 é controlada na superfície celular pelos grupos funcionais (O=C). A hidrofilicidade da superfície celular estava associada com altos níveis dos grupos funcionais [C-(O.N)] e [OH-(C-O-C)], enquanto a hidrofobicidade estava associada com os grupos funcionais [C-(C,H)].
ISSN:1517-8382
1678-4405