Generation, maintenance, and monitoring of gnotobiotic mice
Summary: Gnotobiology has revolutionized the study of microbiota-host interactions. This protocol explains how to generate, maintain, and monitor gnotobiotic mice. Three monitoring methods are presented and compared: bacterial culture, microscopy to visualize the presence (or absence) of bacteria us...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002434 |
| id |
doaj-dffc2629422349d9b5f3c9ef6ccf7312 |
|---|---|
| record_format |
Article |
| spelling |
doaj-dffc2629422349d9b5f3c9ef6ccf73122021-06-21T04:25:35ZengElsevierSTAR Protocols2666-16672021-06-0122100536Generation, maintenance, and monitoring of gnotobiotic miceAmanda Z. Zucoloto0Ian-Ling Yu1Kathy D. McCoy2Braedon McDonald3Department of Critical Care Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; Calvin, Phoebe, and Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; International Microbiome Centre, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; Corresponding authorDepartment of Critical Care Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, CanadaCalvin, Phoebe, and Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; International Microbiome Centre, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, CanadaDepartment of Critical Care Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; Calvin, Phoebe, and Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; International Microbiome Centre, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4A1, Canada; Corresponding authorSummary: Gnotobiology has revolutionized the study of microbiota-host interactions. This protocol explains how to generate, maintain, and monitor gnotobiotic mice. Three monitoring methods are presented and compared: bacterial culture, microscopy to visualize the presence (or absence) of bacteria using Gram staining or DNA staining, and 16S rRNA gene amplification and sequencing. The generation and maintenance of gnotobiotic animals should be performed in a germ-free and gnotobiotic facility to guarantee sterility and precision of gnotobiotic conditions.For complete details on the use and execution of this protocol, please refer to McDonald et al., 2020.http://www.sciencedirect.com/science/article/pii/S2666166721002434SequencingImmunologyMicrobiologyMicroscopyModel OrganismsMolecular Biology |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Amanda Z. Zucoloto Ian-Ling Yu Kathy D. McCoy Braedon McDonald |
| spellingShingle |
Amanda Z. Zucoloto Ian-Ling Yu Kathy D. McCoy Braedon McDonald Generation, maintenance, and monitoring of gnotobiotic mice STAR Protocols Sequencing Immunology Microbiology Microscopy Model Organisms Molecular Biology |
| author_facet |
Amanda Z. Zucoloto Ian-Ling Yu Kathy D. McCoy Braedon McDonald |
| author_sort |
Amanda Z. Zucoloto |
| title |
Generation, maintenance, and monitoring of gnotobiotic mice |
| title_short |
Generation, maintenance, and monitoring of gnotobiotic mice |
| title_full |
Generation, maintenance, and monitoring of gnotobiotic mice |
| title_fullStr |
Generation, maintenance, and monitoring of gnotobiotic mice |
| title_full_unstemmed |
Generation, maintenance, and monitoring of gnotobiotic mice |
| title_sort |
generation, maintenance, and monitoring of gnotobiotic mice |
| publisher |
Elsevier |
| series |
STAR Protocols |
| issn |
2666-1667 |
| publishDate |
2021-06-01 |
| description |
Summary: Gnotobiology has revolutionized the study of microbiota-host interactions. This protocol explains how to generate, maintain, and monitor gnotobiotic mice. Three monitoring methods are presented and compared: bacterial culture, microscopy to visualize the presence (or absence) of bacteria using Gram staining or DNA staining, and 16S rRNA gene amplification and sequencing. The generation and maintenance of gnotobiotic animals should be performed in a germ-free and gnotobiotic facility to guarantee sterility and precision of gnotobiotic conditions.For complete details on the use and execution of this protocol, please refer to McDonald et al., 2020. |
| topic |
Sequencing Immunology Microbiology Microscopy Model Organisms Molecular Biology |
| url |
http://www.sciencedirect.com/science/article/pii/S2666166721002434 |
| work_keys_str_mv |
AT amandazzucoloto generationmaintenanceandmonitoringofgnotobioticmice AT ianlingyu generationmaintenanceandmonitoringofgnotobioticmice AT kathydmccoy generationmaintenanceandmonitoringofgnotobioticmice AT braedonmcdonald generationmaintenanceandmonitoringofgnotobioticmice |
| _version_ |
1721368934139035648 |