DNA secondary structure is influenced by genetic variation and alters susceptibility to <it>de novo </it>translocation

• Main Authors: Tsutsumi Makiko, Yamada Kouji, Ohye Tamae, Kogo Hiroshi, Tong Maoqing, Inagaki Hidehito, Kato Takema, Emanuel Beverly S, Kurahashi Hiroki
• Format: Article
• Language: English
• Published: BMC 2011-09-01
• Series: Molecular Cytogenetics
• Subjects: Polymorphism; Palindrome; Secondary structure; Hairpin structure; Cruciform structure; Breakpoint; Translocation
• Online Access: http://www.molecularcytogenetics.org/content/4/1/18

<p>Abstract</p> <p><b>Background</b></p> <p>Cumulative evidence suggests that DNA secondary structures impact DNA replication, transcription and genomic rearrangements. One of the best studied examples is the recurrent constitutional t(11;22) in humans that is mediated by potentially cruciform-forming sequences at the breakpoints, palindromic AT-rich repeats (PATRRs). We previously demonstrated that polymorphisms of PATRR sequences affect the frequency of <it>de novo </it>t(11;22)s in sperm samples from normal healthy males. These studies were designed to determine whether PATRR polymorphisms affect DNA secondary structure, thus leading to variation in translocation frequency.</p> <p><b>Methods</b></p> <p>We studied the potential for DNA cruciform formation for several PATRR11 polymorphic alleles using mobility shift analysis in gel electrophoresis as well as by direct visualization of the DNA by atomic force microscopy. The structural data for various alleles were compared with the frequency of <it>de novo </it>t(11;22)s the allele produced.</p> <p><b>Results</b></p> <p>The data indicate that the propensity for DNA cruciform structure of each polymorphic allele correlates with the frequency of <it>de novo </it>t(11;22)s produced (r = 0.77, <it>P </it>= 0.01).</p> <p><b>Conclusions</b></p> <p>Although indirect, our results strongly suggest that the PATRR adopts unstable cruciform structures during spermatogenesis that act as translocation hotspots in humans.</p>