A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

Abstract Background Intrabodies enable targeting of proteins in live cells, but generating specific intrabodies against the thousands of proteins in a proteome poses a challenge. We leverage the widespread availability of fluorescently labelled proteins to visualize and manipulate intracellular sign...

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Bibliographic Details
Main Authors: David L. Prole, Colin W. Taylor
Format: Article
Language:English
Published: BMC 2019-05-01
Series:BMC Biology
Subjects:
Cell signalling
Endoplasmic reticulum
Fluorescence microscopy
Fluorescent protein
GFP
Intrabody
Online Access:http://link.springer.com/article/10.1186/s12915-019-0662-4

Internet

http://link.springer.com/article/10.1186/s12915-019-0662-4

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