Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages

Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages

We examined the effect of trimidox (3,4,5-trihydroxybenzamidoxime) on the production of nitric oxide (NO) by lipopolysaccharide (LPS) in mouse RAW 264.7 macrophages. Trimidox (50 – 300 µM) concentration-dependently inhibited NO production by LPS (0.01, 0.1, or 1 µg/ml) after incubation for 24 h. LPS...

Full description

Bibliographic Details
Main Authors: Syu-ichi Kanno, Mai Kakuta, Yasue Kitajima, Yuu Osanai, Kaori Kurauchi, Takaharu Ohtake, Mayuko Ujibe, Kouji Uwai, Mituhiro Takeshita, Masaaki Ishikawa
Format: Article
Language:English
Published: Elsevier 2007-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319342549
id doaj-df8f35e7ff704caa9132d5502894f851
record_format Article
spelling doaj-df8f35e7ff704caa9132d5502894f8512020-11-25T01:20:32ZengElsevierJournal of Pharmacological Sciences1347-86132007-01-011043278281Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 MacrophagesSyu-ichi Kanno0Mai Kakuta1Yasue Kitajima2Yuu Osanai3Kaori Kurauchi4Takaharu Ohtake5Mayuko Ujibe6Kouji Uwai7Mituhiro Takeshita8Masaaki Ishikawa9Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan; Corresponding author. syu-kan@tohoku-pharm.ac.jpDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Pharmaceutics, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Pharmaceutics, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanDepartment of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, JapanWe examined the effect of trimidox (3,4,5-trihydroxybenzamidoxime) on the production of nitric oxide (NO) by lipopolysaccharide (LPS) in mouse RAW 264.7 macrophages. Trimidox (50 – 300 µM) concentration-dependently inhibited NO production by LPS (0.01, 0.1, or 1 µg/ml) after incubation for 24 h. LPS-induced expression of inducible NO synthase (iNOS) and degradation of IκBα were prevented by trimidox. The protective effect against NO production by LPS was not only observed in prior incubation but also later incubation with trimidox until iNOS was activated by LPS. These results suggest that trimidox has a predominantly protective effect against LPS-induced production of NO via iNOS expression. Keywords:: trimidox, nitric oxide, macrophagehttp://www.sciencedirect.com/science/article/pii/S1347861319342549
collection DOAJ
language English
format Article
sources DOAJ
author Syu-ichi Kanno
Mai Kakuta
Yasue Kitajima
Yuu Osanai
Kaori Kurauchi
Takaharu Ohtake
Mayuko Ujibe
Kouji Uwai
Mituhiro Takeshita
Masaaki Ishikawa
spellingShingle Syu-ichi Kanno
Mai Kakuta
Yasue Kitajima
Yuu Osanai
Kaori Kurauchi
Takaharu Ohtake
Mayuko Ujibe
Kouji Uwai
Mituhiro Takeshita
Masaaki Ishikawa
Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages
Journal of Pharmacological Sciences
author_facet Syu-ichi Kanno
Mai Kakuta
Yasue Kitajima
Yuu Osanai
Kaori Kurauchi
Takaharu Ohtake
Mayuko Ujibe
Kouji Uwai
Mituhiro Takeshita
Masaaki Ishikawa
author_sort Syu-ichi Kanno
title Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages
title_short Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages
title_full Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages
title_fullStr Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages
title_full_unstemmed Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages
title_sort inhibitory effect of trimidox on lipopolysaccharide-induced nitric oxide production in raw 264.7 macrophages
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2007-01-01
description We examined the effect of trimidox (3,4,5-trihydroxybenzamidoxime) on the production of nitric oxide (NO) by lipopolysaccharide (LPS) in mouse RAW 264.7 macrophages. Trimidox (50 – 300 µM) concentration-dependently inhibited NO production by LPS (0.01, 0.1, or 1 µg/ml) after incubation for 24 h. LPS-induced expression of inducible NO synthase (iNOS) and degradation of IκBα were prevented by trimidox. The protective effect against NO production by LPS was not only observed in prior incubation but also later incubation with trimidox until iNOS was activated by LPS. These results suggest that trimidox has a predominantly protective effect against LPS-induced production of NO via iNOS expression. Keywords:: trimidox, nitric oxide, macrophage
url http://www.sciencedirect.com/science/article/pii/S1347861319342549
work_keys_str_mv AT syuichikanno inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT maikakuta inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT yasuekitajima inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT yuuosanai inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT kaorikurauchi inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT takaharuohtake inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT mayukoujibe inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT koujiuwai inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT mituhirotakeshita inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
AT masaakiishikawa inhibitoryeffectoftrimidoxonlipopolysaccharideinducednitricoxideproductioninraw2647macrophages
_version_ 1725133698052915200

Similar Items