Comparison of dengue infection in human mononuclear leukocytes with mosquito C6/36 and mammalian Vero cells using flow cytometry to detect virus antigen

Comparison of dengue infection in human mononuclear leukocytes with mosquito C6/36 and mammalian Vero cells using flow cytometry to detect virus antigen

Fluorescent activated cell sorter (FACS) analysis is useful for the detection of cellular surface antigens and intracellular proteins. We used this methodology in order to detect and quantify dengue antigens in highly susceptible cells such as clone C6/36 (Aedes albopictus) and Vero cells (green mon...

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Bibliographic Details
Main Authors: Farid FO von Sydow, Marta A Santiago, Patricia C Neves-Souza, Denise IS Cerqueira, Adriana S Gouvea, Maryrose FH Lavatori, Álvaro L Bertho, Claire F Kubelka
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2000-08-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
dengue virus
C6/36
Vero cells
mononuclear leukocytes
flow cytometry
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000400007

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