ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer

ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer

A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, U...

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Bibliographic Details
Main Authors: Leonardo A. Calderon, Rodrigo G. Stábeli, Juliana P. Zuliani, Andreimar M. Soares, Anderson M. Kayano, Rodrigo S. Silva, Kayena D. Zaqueo, Gustavo H. M. F. Souza, Cleópatra A. S. Caldeira, Antonio Coutinho-Neto
Format: Article
Language:English
Published: MDPI AG 2013-02-01
Series:Toxins
Subjects:
bioactive peptide
BPP
pyroglutamic acid
pyrrolidonecarboxylic acid
de novo peptide sequencing
Online Access:http://www.mdpi.com/2072-6651/5/2/327
Description
Summary:A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12.
ISSN:2072-6651

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