A broadly reactive one-step SYBR Green I real-time RT-PCR assay for rapid detection of murine norovirus.

A broadly reactive one-step SYBR Green I real-time RT-PCR assay for rapid detection of murine norovirus.

A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1-ORF2 junction of MNV. The broad reactivit...

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Bibliographic Details
Main Authors: Ken-Ichi Hanaki, Fumio Ike, Ayako Kajita, Wataru Yasuno, Misato Yanagiba, Motoki Goto, Kouji Sakai, Yasushi Ami, Shigeru Kyuwa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4029972?pdf=render

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