Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract

This study investigated the use of microencapsulated bile salt hydrolase (BSH) overproducing Lactobacillus plantarum 80 cells for oral delivery applications using a dynamic computer-controlled model simulating the human gastrointestinal (GI) tract. Bile salt deconjugation rates for microencapsulated...

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Main Authors:	Christopher Martoni, Jasmine Bhathena, Mitchell Lawrence Jones, Aleksandra Malgorzata Urbanska, Hongmei Chen, Satya Prakash
Format:	Article
Language:	English
Published:	Hindawi Limited 2007-01-01
Series:	Journal of Biomedicine and Biotechnology
Online Access:	http://dx.doi.org/10.1155/2007/13684

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spelling	doaj-df1b53f76e8a417a9a556a56abc3ed4c2020-11-25T02:15:25ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512007-01-01200710.1155/2007/1368413684Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI TractChristopher Martoni0Jasmine Bhathena1Mitchell Lawrence Jones2Aleksandra Malgorzata Urbanska3Hongmei Chen4Satya Prakash5Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, CanadaBiomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, CanadaBiomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, CanadaBiomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, CanadaBiomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, CanadaBiomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, CanadaThis study investigated the use of microencapsulated bile salt hydrolase (BSH) overproducing Lactobacillus plantarum 80 cells for oral delivery applications using a dynamic computer-controlled model simulating the human gastrointestinal (GI) tract. Bile salt deconjugation rates for microencapsulated BSH overproducing cells were 4.87 ± 0.28 μmol/g microcapsule/h towards glycoconjugates and 0.79 ± 0.15 μmol/g microcapsule/h towards tauroconjugates in the simulated intestine, a significant (P< .05) increase over microencapsulated wild-type cells. Microcapsules protected the encased cells in the simulated stomach prior to intestinal release, maintaining cell viability above 109 cfu/mL at pH 2.5 and 3.0 and above 106 cfu/mL at pH 2.0 after 2-hour residence times. In the simulated intestine, encased cell viability was maintained above 1010 cfu/mL after 3, 6, and 12-hour residence times in bile concentrations up to 1.0%. Results show that microencapsulation has potential in the oral delivery of live BSH active bacterial cells. However, in vivo testing is required.http://dx.doi.org/10.1155/2007/13684
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Christopher Martoni Jasmine Bhathena Mitchell Lawrence Jones Aleksandra Malgorzata Urbanska Hongmei Chen Satya Prakash
spellingShingle	Christopher Martoni Jasmine Bhathena Mitchell Lawrence Jones Aleksandra Malgorzata Urbanska Hongmei Chen Satya Prakash Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract Journal of Biomedicine and Biotechnology
author_facet	Christopher Martoni Jasmine Bhathena Mitchell Lawrence Jones Aleksandra Malgorzata Urbanska Hongmei Chen Satya Prakash
author_sort	Christopher Martoni
title	Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract
title_short	Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract
title_full	Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract
title_fullStr	Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract
title_full_unstemmed	Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract
title_sort	investigation of microencapsulated bsh active lactobacillus in the simulated human gi tract
publisher	Hindawi Limited
series	Journal of Biomedicine and Biotechnology
issn	1110-7243 1110-7251
publishDate	2007-01-01
description	This study investigated the use of microencapsulated bile salt hydrolase (BSH) overproducing Lactobacillus plantarum 80 cells for oral delivery applications using a dynamic computer-controlled model simulating the human gastrointestinal (GI) tract. Bile salt deconjugation rates for microencapsulated BSH overproducing cells were 4.87 ± 0.28 μmol/g microcapsule/h towards glycoconjugates and 0.79 ± 0.15 μmol/g microcapsule/h towards tauroconjugates in the simulated intestine, a significant (P< .05) increase over microencapsulated wild-type cells. Microcapsules protected the encased cells in the simulated stomach prior to intestinal release, maintaining cell viability above 109 cfu/mL at pH 2.5 and 3.0 and above 106 cfu/mL at pH 2.0 after 2-hour residence times. In the simulated intestine, encased cell viability was maintained above 1010 cfu/mL after 3, 6, and 12-hour residence times in bile concentrations up to 1.0%. Results show that microencapsulation has potential in the oral delivery of live BSH active bacterial cells. However, in vivo testing is required.
url	http://dx.doi.org/10.1155/2007/13684
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Investigation of Microencapsulated BSH Active Lactobacillus in the Simulated Human GI Tract

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