Construction of a Plasmid Vector for Expression of Bacteriocin N15-encoding Gene and Effect of Engineered Bacteria on Enterococcus Faecalis
A 6.09-kb plasmids vector pOri253 was constructed from the plasmid pIL253 (5.2 kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid pOri253 conferred erythromycinresistance in both Escherichia coli and Enterococcus faecalis. It has a unique site for each of EcoRI,...
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| Format: | Article |
| Language: | English |
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Walailak University
2011-12-01
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| Series: | Walailak Journal of Science and Technology |
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| Online Access: | http://wjst.wu.ac.th/index.php/wjst/article/view/163 |