Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging

Accumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD...

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Main Authors: Aleksandra Jankovic, Luciano Saso, Aleksandra Korac, Bato Korac
Format: Article
Language:English
Published: Hindawi Limited 2019-01-01
Series:Oxidative Medicine and Cellular Longevity
Online Access:http://dx.doi.org/10.1155/2019/2471312
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spelling doaj-deb1862531344bb7b7768dc9774db99e2020-11-25T00:28:48ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942019-01-01201910.1155/2019/24713122471312Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological AgingAleksandra Jankovic0Luciano Saso1Aleksandra Korac2Bato Korac3Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, SerbiaDepartment of Physiology and Pharmacology “Vittorio Erspamer”, Faculty of Pharmacy and Medicine, Sapienza University of Rome, ItalyFaculty of Biology, University of Belgrade, SerbiaInstitute for Biological Research “Sinisa Stankovic”, University of Belgrade, SerbiaAccumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD) enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), thioredoxin reductase (TR), and methionine sulfoxide reductase A (MsrA), and the markers of oxidative damage of biomolecules—4-hydroxynonenal (HNE) and 8-oxoguanine (8-oxoG)—were examined in the rat skin during life (from 3 days to 21 months). As compared to adult 3-month-old skin, higher activities of CAT, GSH-Px, and GR and a decline in expression of MsrA are found in 21-month-old skin. These changes correspond to degenerative changes at structural and ultrastructural levels in epidermal and dermal compartments, low proliferation capacity, and higher levels of HNE-modified protein aldehydes (particularly in basal lamina) and 8-oxoG positivity in nuclei and mitochondria in the sebaceous glands and root sheath. In 3-day-old skin, higher activities of AD enzymes (SOD, CAT, GR, and TR) and MsrA expression correspond to intensive postnatal development and proliferation. In contrast to 21-month-old skin, a high level of HNE in young skin is not accompanied by 8-oxoG positivity or any morphological disturbances. Observed results indicate that increased activity of AD enzymes in elderly rat skin represents the compensatory response to accumulated oxidative damage of DNA and proteins, accompanied by attenuated repair and proliferative capacity, but in young rats the redox changes are necessary and inherent with processes which occur during postnatal skin development. Мorphological and ultrastructurаl changes are in line with the redox profile in the skin of young and old rats.http://dx.doi.org/10.1155/2019/2471312
collection DOAJ
language English
format Article
sources DOAJ
author Aleksandra Jankovic
Luciano Saso
Aleksandra Korac
Bato Korac
spellingShingle Aleksandra Jankovic
Luciano Saso
Aleksandra Korac
Bato Korac
Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
Oxidative Medicine and Cellular Longevity
author_facet Aleksandra Jankovic
Luciano Saso
Aleksandra Korac
Bato Korac
author_sort Aleksandra Jankovic
title Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
title_short Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
title_full Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
title_fullStr Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
title_full_unstemmed Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
title_sort relation of redox and structural alterations of rat skin in the function of chronological aging
publisher Hindawi Limited
series Oxidative Medicine and Cellular Longevity
issn 1942-0900
1942-0994
publishDate 2019-01-01
description Accumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD) enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), thioredoxin reductase (TR), and methionine sulfoxide reductase A (MsrA), and the markers of oxidative damage of biomolecules—4-hydroxynonenal (HNE) and 8-oxoguanine (8-oxoG)—were examined in the rat skin during life (from 3 days to 21 months). As compared to adult 3-month-old skin, higher activities of CAT, GSH-Px, and GR and a decline in expression of MsrA are found in 21-month-old skin. These changes correspond to degenerative changes at structural and ultrastructural levels in epidermal and dermal compartments, low proliferation capacity, and higher levels of HNE-modified protein aldehydes (particularly in basal lamina) and 8-oxoG positivity in nuclei and mitochondria in the sebaceous glands and root sheath. In 3-day-old skin, higher activities of AD enzymes (SOD, CAT, GR, and TR) and MsrA expression correspond to intensive postnatal development and proliferation. In contrast to 21-month-old skin, a high level of HNE in young skin is not accompanied by 8-oxoG positivity or any morphological disturbances. Observed results indicate that increased activity of AD enzymes in elderly rat skin represents the compensatory response to accumulated oxidative damage of DNA and proteins, accompanied by attenuated repair and proliferative capacity, but in young rats the redox changes are necessary and inherent with processes which occur during postnatal skin development. Мorphological and ultrastructurаl changes are in line with the redox profile in the skin of young and old rats.
url http://dx.doi.org/10.1155/2019/2471312
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