Transcriptomic and Genomic Testing to Guide Individualized Treatment in Chemoresistant Gastric Cancer Case

• Main Authors: Alexey Moisseev, Eugene Albert, Dan Lubarsky, David Schroeder, Jeffrey Clark
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Biomedicines
• Subjects: gastric cancer; stomach adenocarcinoma; genomic test; molecular pathway analysis; immunotherapy; platform comparison; mutation; expression analysis; companion diagnostics
• Online Access: https://www.mdpi.com/2227-9059/8/3/67

Gastric cancer is globally the fifth leading cause of cancer death. We present a case report describing the unique genomic characteristics of an Epstein&#8722;Barr virus-negative gastric cancer with esophageal invasion and regional lymph node metastasis. Genomic tests were performed first with the stomach biopsy using platforms FoundationOne, OncoDNA, and Oncopanel at Dana Farber Institute. Following neoadjuvant chemotherapy, residual tumor was resected and the stomach and esophageal residual tumor samples were compared with the initial biopsy by whole exome sequencing and molecular pathway analysis platform Oncobox. Copy number variation profiling perfectly matched the whole exome sequencing results. A moderate agreement was seen between the diagnostic platforms in finding mutations in the initial biopsy. Final data indicate somatic activating mutation Q546K in <i>PIK3CA</i> gene, somatic frameshifts in <i>PIH1D1</i> and <i>FBXW7</i> genes, stop-gain in <i>TP53BP1</i>, and a few somatic mutations of unknown significance. RNA sequencing analysis revealed upregulated expressions of <i>MMP7, MMP9, BIRC5,</i> and <i>PD-L1</i> genes and strongly differential regulation of several molecular pathways linked with the mutations identified. According to test results, the patient received immunotherapy with anti-PD1 therapy and is now free of disease for 2 years. Our data suggest that matched tumor and normal tissue analyses have a considerable advantage over tumor biopsy-only genomic tests in stomach cancer.