Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis
<p>Abstract</p> <p>Background</p> <p>The generation of focused mutant libraries at hotspot residues is an important strategy in directed protein evolution. Existing methods, such as combinatorial active site testing and residual coupling analysis, depend primarily on th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-10-01
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| Series: | BMC Bioinformatics |
| Online Access: | http://www.biomedcentral.com/1471-2105/13/263 |