Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, <i>Viscum album</i> L. (White Berry Mistletoe), Derived from Different Host Trees

• Main Authors: Mahak Majeed, Tanveer Bilal Pirzadah, Manzoor Ahmad Mir, Khalid Rehman Hakeem, Hesham F. Alharby, Hameed Alsamadany, Atif A. Bamagoos, Reiaz Ul Rehman
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Plants
• Subjects: antioxidant; extraction; epiphyte phytochemical; plant extract
• Online Access: https://www.mdpi.com/2223-7747/10/6/1191

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte <i>Viscum album</i> L. harvested from three tree species, namely, <i>Populus ciliata</i> L, <i>Ulmus villosa</i> L., and <i>Juglans regia</i> L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (^•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host <i>Juglans regia</i> L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in <i>Viscum album</i> L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.