Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice

AMP-activated protein kinase (AMPK) activation is an established treatment for diabetes. Here the effects of anthocyanin extract from blood orange juice, i.e. cyanidin-3-O-β-glucoside (C3G) and its secondary metabolite protocatechuic acid (PCA), on AMPK signalling in murine hepatic cell line and in...

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Main Authors: Veena Talagavadi, Paolo Rapisarda, Fabio Galvano, Piergiuseppe Pelicci, Marco Giorgio
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Journal of Functional Foods
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464615006118
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spelling doaj-ddfd4f21601d42db8ffae7e149f2f7af2021-04-29T04:45:56ZengElsevierJournal of Functional Foods1756-46462016-03-0121338348Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in miceVeena Talagavadi0Paolo Rapisarda1Fabio Galvano2Piergiuseppe Pelicci3Marco Giorgio4Experimental Oncology Department, European Institute of Oncology, Via Adamello 16, Milano, ItalyAgrumiculture Experimental Institute (ISA), Corso Savoia 190, 95024 Acireale, ItalyInternal Medicine Department, University of Catania, Via Santa Sofia 78, Catania, ItalyExperimental Oncology Department, European Institute of Oncology, Via Adamello 16, Milano, ItalyExperimental Oncology Department, European Institute of Oncology, Via Adamello 16, Milano, Italy; Corresponding author. Experimental Oncology Department, European Institute of Oncology, Via Adamello 16, 20139 Milano, Italy. Tel.: +39 02 94355040; fax: +39 02 9437 5990.AMP-activated protein kinase (AMPK) activation is an established treatment for diabetes. Here the effects of anthocyanin extract from blood orange juice, i.e. cyanidin-3-O-β-glucoside (C3G) and its secondary metabolite protocatechuic acid (PCA), on AMPK signalling in murine hepatic cell line and in the liver of C57BL/6 mice were investigated. Results showed for the first time that C3G and PCA activate AMPK and suppress its downstream kinase mTOR/S6K both in vitro and in vivo systems. Then, C3G and particularly PCA increased expression of glut 1 and glut 4 in the liver and improved glucose tolerance in normal and obese mice. Finally, the consumption of blood orange juice was observed to increase insulin sensitivity in mice. These findings indicate that C3G and PCA are natural AMPK activators and dietary consumption of food sources of C3G and PCA improves glucose homeostasis. Mainly, blood orange juice has beneficial effect for type 2 diabetes.http://www.sciencedirect.com/science/article/pii/S1756464615006118Blood orangesAnthocyaninAMPKmTORGlucose homeostasisLaboratory mice
collection DOAJ
language English
format Article
sources DOAJ
author Veena Talagavadi
Paolo Rapisarda
Fabio Galvano
Piergiuseppe Pelicci
Marco Giorgio
spellingShingle Veena Talagavadi
Paolo Rapisarda
Fabio Galvano
Piergiuseppe Pelicci
Marco Giorgio
Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice
Journal of Functional Foods
Blood oranges
Anthocyanin
AMPK
mTOR
Glucose homeostasis
Laboratory mice
author_facet Veena Talagavadi
Paolo Rapisarda
Fabio Galvano
Piergiuseppe Pelicci
Marco Giorgio
author_sort Veena Talagavadi
title Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice
title_short Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice
title_full Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice
title_fullStr Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice
title_full_unstemmed Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice
title_sort cyanidin-3-o-β-glucoside and protocatechuic acid activate ampk/mtor/s6k pathway and improve glucose homeostasis in mice
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2016-03-01
description AMP-activated protein kinase (AMPK) activation is an established treatment for diabetes. Here the effects of anthocyanin extract from blood orange juice, i.e. cyanidin-3-O-β-glucoside (C3G) and its secondary metabolite protocatechuic acid (PCA), on AMPK signalling in murine hepatic cell line and in the liver of C57BL/6 mice were investigated. Results showed for the first time that C3G and PCA activate AMPK and suppress its downstream kinase mTOR/S6K both in vitro and in vivo systems. Then, C3G and particularly PCA increased expression of glut 1 and glut 4 in the liver and improved glucose tolerance in normal and obese mice. Finally, the consumption of blood orange juice was observed to increase insulin sensitivity in mice. These findings indicate that C3G and PCA are natural AMPK activators and dietary consumption of food sources of C3G and PCA improves glucose homeostasis. Mainly, blood orange juice has beneficial effect for type 2 diabetes.
topic Blood oranges
Anthocyanin
AMPK
mTOR
Glucose homeostasis
Laboratory mice
url http://www.sciencedirect.com/science/article/pii/S1756464615006118
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