Analysis of PAI-1 gene polymorphism of dust mite allergens in children

• Main Author: Feng Li
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-02-01
• Series: Journal of Hainan Medical University
• Subjects: Dust mite allergy in children; PAI-1 gene; Polymorphism.
• Online Access: http://www.jhmuweb.net/PDF/201603/23.pdf

Objective: To explore PAI-1 gene polymorphism of dust mite allergens in children. Methods: A total of 160 allergic children who were admitted in our hospital from June, 2013 to June, 2015 were included in the study and performed with the dust mite allergy test, among which 80 cases with the positive results were served as the observation group, while 80 healthy children with the negative results were served as the control group. ASPCR was used to analyze PAI-1 gene polymorphism. ELISA was used to detect the content of plasma PAI-1. Results: The detection of genotype frequency goodness fit showed that the genotypes in the two groups reached Hardy-Weinberg balance. PAI-1 4G/4G genotype carrying frequency in the observation group was significantly higher than that in the control group (P<0.05). 5G/5G genotype carrying frequency in the observation group was significantly lower than that in the control group (P<0.05), while 4G allele carrying frequency was significantly higher than that in the control group (P<0.05). The content of plasma PAI-1 in the observation group with 4G/4G was significantly higher than that in patients with 4G/5G and 5G/5G. Conclusions: PAI-1 gene polymorphism is probably the risk factor for dust mite allergy. Deep insight of the structure and function of PAI-1 can guide the clinician to prevent and intervene the dust mite allergy in children.