Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum

Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum

Abstract Background The genome-editing tool CRISPR/Cas9 has revolutionized gene manipulation by providing an efficient method to generate targeted mutations. This technique deploys the Cas9 endonuclease and a guide RNA (sgRNA) which interact to form a Cas9-sgRNA complex that initiates gene editing t...

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Bibliographic Details
Main Authors: Haseena Khan, Megan C. McDonald, Simon J. Williams, Peter S. Solomon
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Fungal Biology and Biotechnology
Subjects:
CRISPR/Cas9
Gene editing
Ribonuclear protein complex
Parastagonospora nodorum
Online Access:http://link.springer.com/article/10.1186/s40694-020-00094-0

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