Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury

Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury

qRT-PCR still remains the most widely used method for quantifying gene expression levels, although newer technologies such as next generation sequencing are becoming increasingly popular. A critical, yet often underappreciated, problem when analysing qRT-PCR data is the selection of suitable referen...

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Bibliographic Details
Main Authors:	Athanassios Fragoulis, Kristina Biller, Stephanie Fragoulis, Dennis Lex, Stefan Uhlig, Lucy Kathleen Reiss
Format:	Article
Language:	English
Published:	MDPI AG 2021-07-01
Series:	International Journal of Molecular Sciences
Subjects:	primer validation reference gene establishment qPCR normalisation ALI mouse models VILI IPL
Online Access:	https://www.mdpi.com/1422-0067/22/15/7853

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