Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines

Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines

Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometr...

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Main Authors: Miroslava Rabajdová, Ivana Špaková, Zuzana Klepcová, Lukáš Smolko, Michaela Abrahamovská, Peter Urdzík, Mária Mareková
Format: Article
Language:English
Published: Nature Publishing Group 2021-09-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-98512-9
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spelling doaj-ddc14f71d5fc4288a7f5bc1a69c646e62021-10-03T11:31:25ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111310.1038/s41598-021-98512-9Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell linesMiroslava Rabajdová0Ivana Špaková1Zuzana Klepcová2Lukáš Smolko3Michaela Abrahamovská4Peter Urdzík5Mária Mareková6Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik UniversityDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik UniversityDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik UniversityDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik UniversityDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik UniversityDepartment of Gynaecology and Obstetrics, Faculty of Medicine, Pavol Jozef Šafárik UniversityDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik UniversityAbstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on control immortalized human endometrial stromal cell line (hTERT) was performed using xCELLigence technology for approximately 72 h following the treatment with Zn-Nif as well as cell viability Trypan Blue Assay. 12Z cell line proliferated more slowly compared to unaffected cells, whereas hTERT cells did not show similar behavior after treatment. The complex probably reduces the effect of pro-inflammatory pathways due to the effect of NSAID, while presence of zinc might reduce the level of ROS and regulate ER2 levels and MMP activity. The observed effects and high selectivity for rapidly proliferating cells with increased inflammatory activity suggest a good prognosis of successful decrease of endometriosis stage with this complex.https://doi.org/10.1038/s41598-021-98512-9
collection DOAJ
language English
format Article
sources DOAJ
author Miroslava Rabajdová
Ivana Špaková
Zuzana Klepcová
Lukáš Smolko
Michaela Abrahamovská
Peter Urdzík
Mária Mareková
spellingShingle Miroslava Rabajdová
Ivana Špaková
Zuzana Klepcová
Lukáš Smolko
Michaela Abrahamovská
Peter Urdzík
Mária Mareková
Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
Scientific Reports
author_facet Miroslava Rabajdová
Ivana Špaková
Zuzana Klepcová
Lukáš Smolko
Michaela Abrahamovská
Peter Urdzík
Mária Mareková
author_sort Miroslava Rabajdová
title Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_short Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_full Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_fullStr Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_full_unstemmed Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
title_sort zinc(ii) niflumato complex effects on mmp activity and gene expression in human endometrial cell lines
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-09-01
description Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on control immortalized human endometrial stromal cell line (hTERT) was performed using xCELLigence technology for approximately 72 h following the treatment with Zn-Nif as well as cell viability Trypan Blue Assay. 12Z cell line proliferated more slowly compared to unaffected cells, whereas hTERT cells did not show similar behavior after treatment. The complex probably reduces the effect of pro-inflammatory pathways due to the effect of NSAID, while presence of zinc might reduce the level of ROS and regulate ER2 levels and MMP activity. The observed effects and high selectivity for rapidly proliferating cells with increased inflammatory activity suggest a good prognosis of successful decrease of endometriosis stage with this complex.
url https://doi.org/10.1038/s41598-021-98512-9
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