Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphami...

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Main Authors: Sabran Affidah, Kumolosasi Endang, Jantan Ibrahim
Format: Article
Language:English
Published: Sciendo 2019-03-01
Series:Acta Pharmaceutica
Subjects:
annexin a1
apoptosis
cell cycle
flow cytometry
human leukemic cell lines
Online Access:https://doi.org/10.2478/acph-2019-0005
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spelling doaj-ddb307c99623495291a3d18ab90487042021-09-06T19:40:59ZengSciendoActa Pharmaceutica1846-95582019-03-01691758610.2478/acph-2019-0005acph-2019-0005Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell linesSabran Affidah0Kumolosasi Endang1Jantan Ibrahim2Drug and Herbal Research Centre Faculty of Pharmacy, University Kebangsaan Malaysia, Kuala LumpurMalaysiaDrug and Herbal Research Centre Faculty of Pharmacy, University Kebangsaan Malaysia, Kuala LumpurMalaysiaSchool of Pharmacy, Taylor’s University, Lakeside Campus, 47500Subang Jaya, Selangor, MalaysiaRecent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5µM, ANXA1 induced significant apoptosis in K562 (p ≤ 0.001) and U937 (p ≤ 0.05) cells, with EC50 values of 3.6 and 3.8 µM, respectively. In Jurkat cells, induction was not significant (EC50, 17.0 µM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with p ≤ 0.001 for both, and (p ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.https://doi.org/10.2478/acph-2019-0005annexin a1apoptosiscell cycleflow cytometryhuman leukemic cell lines
collection DOAJ
language English
format Article
sources DOAJ
author Sabran Affidah
Kumolosasi Endang
Jantan Ibrahim
spellingShingle Sabran Affidah
Kumolosasi Endang
Jantan Ibrahim
Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
Acta Pharmaceutica
annexin a1
apoptosis
cell cycle
flow cytometry
human leukemic cell lines
author_facet Sabran Affidah
Kumolosasi Endang
Jantan Ibrahim
author_sort Sabran Affidah
title Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
title_short Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
title_full Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
title_fullStr Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
title_full_unstemmed Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
title_sort effects of annexin a1 on apoptosis and cell cycle arrest in human leukemic cell lines
publisher Sciendo
series Acta Pharmaceutica
issn 1846-9558
publishDate 2019-03-01
description Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5µM, ANXA1 induced significant apoptosis in K562 (p ≤ 0.001) and U937 (p ≤ 0.05) cells, with EC50 values of 3.6 and 3.8 µM, respectively. In Jurkat cells, induction was not significant (EC50, 17.0 µM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with p ≤ 0.001 for both, and (p ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.
topic annexin a1
apoptosis
cell cycle
flow cytometry
human leukemic cell lines
url https://doi.org/10.2478/acph-2019-0005
work_keys_str_mv AT sabranaffidah effectsofannexina1onapoptosisandcellcyclearrestinhumanleukemiccelllines
AT kumolosasiendang effectsofannexina1onapoptosisandcellcyclearrestinhumanleukemiccelllines
AT jantanibrahim effectsofannexina1onapoptosisandcellcyclearrestinhumanleukemiccelllines
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