Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia

• Main Authors: Sri Wedhastri, Dinar Mindrati Fardhani, Siti Kabirun, Jaka Widada, Donny Widianto, Rusdi Evizal, Irfan Dwidya Prijambada
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7879

<div>Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form root</div><div>nodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type of</div><div>agroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera,</div><div>Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodule</div><div>in Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifH</div><div>and nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48a</div><div>displayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulated</div><div>Glycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species for</div><div>Glycine max possesing the positive copy of nodA gene.</div><div><br /></div><div>Keywords : Legume Nodulating Bacteria, shrub agroecosystem, Achromobacter xylosoxidans, nodA, Glycine max</div>