Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization

It is known that the pathological course of pregnancy is associated with a disturbance in the interdependent “mother-fetus” complex and leads to specific clinical consequences at the molecular-cellular, tissue, organ, organismal and population levels of organization. In case of violation of placenta...

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Main Authors: Agarkov Alexander, Grudeva Elena, Agarkov Nikolay, Pisarenko Natalia, Belugin Nikolay
Format: Article
Language:English
Published: EDP Sciences 2021-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/49/e3sconf_interagromash2021_02003.pdf
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spelling doaj-dd9cb9c97ee1491baa69395b7abd60bd2021-07-07T11:30:49ZengEDP SciencesE3S Web of Conferences2267-12422021-01-012730200310.1051/e3sconf/202127302003e3sconf_interagromash2021_02003Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunizationAgarkov Alexander0Grudeva Elena1Agarkov Nikolay2Pisarenko Natalia3Belugin Nikolay4Stavropol State Agrarian UniversityStavropol State Agrarian UniversityStavropol State Agrarian UniversityStavropol State Agrarian UniversityStavropol State Agrarian UniversityIt is known that the pathological course of pregnancy is associated with a disturbance in the interdependent “mother-fetus” complex and leads to specific clinical consequences at the molecular-cellular, tissue, organ, organismal and population levels of organization. In case of violation of placentation (placental barrier), a state of immune conflict occurs, characterized by an antigen-antibody reaction, carried out through the placenta (in relation to the fetus), or through colostrum after birth (in relation to the newborn offspring). Therefore, immunological relations at the population level should be considered both the consequences of the action of the maternal organism’s antigens on the fetus, and the effects of the allogeneic action of the fetus on the mother’s body. According to the results of the experiment, the placentas of 10 sows of Large White breed were used as the material, which showed signs of isoimmunization to the resulting offspring. For histological examination, immediately after delivery, pieces of placenta up to 0.5 cm thick were selected, which were fixed in a 10% aqueous solution of neutral formalin. The fixed material after wiring through alcohols of increasing concentration, xylene, xylene-paraffin, was poured into paraffin. The obtained preparations were stained with conventional methods-hematoxylin and eosin. d (fibrinoid masses).https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/49/e3sconf_interagromash2021_02003.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Agarkov Alexander
Grudeva Elena
Agarkov Nikolay
Pisarenko Natalia
Belugin Nikolay
spellingShingle Agarkov Alexander
Grudeva Elena
Agarkov Nikolay
Pisarenko Natalia
Belugin Nikolay
Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
E3S Web of Conferences
author_facet Agarkov Alexander
Grudeva Elena
Agarkov Nikolay
Pisarenko Natalia
Belugin Nikolay
author_sort Agarkov Alexander
title Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
title_short Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
title_full Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
title_fullStr Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
title_full_unstemmed Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
title_sort evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization
publisher EDP Sciences
series E3S Web of Conferences
issn 2267-1242
publishDate 2021-01-01
description It is known that the pathological course of pregnancy is associated with a disturbance in the interdependent “mother-fetus” complex and leads to specific clinical consequences at the molecular-cellular, tissue, organ, organismal and population levels of organization. In case of violation of placentation (placental barrier), a state of immune conflict occurs, characterized by an antigen-antibody reaction, carried out through the placenta (in relation to the fetus), or through colostrum after birth (in relation to the newborn offspring). Therefore, immunological relations at the population level should be considered both the consequences of the action of the maternal organism’s antigens on the fetus, and the effects of the allogeneic action of the fetus on the mother’s body. According to the results of the experiment, the placentas of 10 sows of Large White breed were used as the material, which showed signs of isoimmunization to the resulting offspring. For histological examination, immediately after delivery, pieces of placenta up to 0.5 cm thick were selected, which were fixed in a 10% aqueous solution of neutral formalin. The fixed material after wiring through alcohols of increasing concentration, xylene, xylene-paraffin, was poured into paraffin. The obtained preparations were stained with conventional methods-hematoxylin and eosin. d (fibrinoid masses).
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/49/e3sconf_interagromash2021_02003.pdf
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