Identification of Aeromonas hydrophila infection with specific monoclonal antibodies

• Main Author: Paisarn Sithigorngul
• Format: Article
• Language: English
• Published: Maejo University 2007-08-01
• Series: Maejo International Journal of Science and Technology
• Subjects: Aeromonas hydrophila; dot blotting; immunohistochemistry; monoclonal antibody; lipopolysaccharide (LPS); Western blotting.
• Online Access: http://www.mijst.mju.ac.th/vol1/107-119.pdf

Whole cell of Aeromonas hydrophila 1234 was used for immunization to produce monoclonal antibodies (MAbs). Three different groups of MAbs specific to Aeromonas were obtained. The first group of MAbs demonstrated high specificity and bound to the A. hydrophila 1234 only but did not bind to the other two A. hydrophila isolates. This group of MAbs bound to a series of lipo-polysaccharides (LPS) with molecular masses range from 10 to190 kDa. The second group of antibodies recognized A. hydrophila 1234 and 2798 isolates, and bound to a series of LPS with molecular masses range from 5-200 kDa. The third group of MAbs recognized all three isolates of A. hydrophila and two isolates of A. sobria, and lightly bound to A. caviae. This group of MAbs also bound to an unknown protein with molecular mass of 20 kDa. The MAbs in group 1 and group 2 can be used to detect the bacteria in tissues by immunohistochemistry. Both groups of MAbs bound to LPS at different sites in which the MAbs in group 2 bound to the side chain of O antigen while the MAbs in group 1 bound to the polymerization site at the core of oligosaccharide. All of the MAbs can be used to identify Aeromonas by dot blotting with the sensitivity range from 103-104 CFU/l. This study demonstrated a convenient immunological tool that can be used for simple and accurate identification of A. hydrophila, as well as for diagnosis of the A. hydrophila infection in animals. This immunological tool can replace costly and laborious biochemical tests.