Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>

<i>Background</i>: Insect vector control is facing the challenges of resistance development and environmental hazards caused by synthetic pesticides. This has led to a considerable market opportunity for botanical insecticides. In this scenario, our study investigated the potential of se...

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Main Authors: Mohammad Reza Youssefi, Mohaddeseh Abouhosseini Tabari, Aryan Esfandiari, Sohrab Kazemi, Ali Akbar Moghadamnia, Stefania Sut, Stefano Dall’Acqua, Giovanni Benelli, Filippo Maggi
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/24/10/1867
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spelling doaj-dd6d7a0694bc49298789707f427516972020-11-24T21:29:03ZengMDPI AGMolecules1420-30492019-05-012410186710.3390/molecules24101867molecules24101867Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>Mohammad Reza Youssefi0Mohaddeseh Abouhosseini Tabari1Aryan Esfandiari2Sohrab Kazemi3Ali Akbar Moghadamnia4Stefania Sut5Stefano Dall’Acqua6Giovanni Benelli7Filippo Maggi8Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol 484, IranFaculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol 46131-46391, IranYoung Researchers and Elite Club, Babol Branch, Islamic Azad University, Babol 484, IranCellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol 47176-47745, IranCellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol 47176-47745, IranDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, 35139 Padova, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, ItalySchool of Pharmacy, University of Camerino, 62032 Camerino, Italy<i>Background</i>: Insect vector control is facing the challenges of resistance development and environmental hazards caused by synthetic pesticides. This has led to a considerable market opportunity for botanical insecticides. In this scenario, our study investigated the potential of selected bioactive monoterpenoids, carvacrol and thymol, as safe and effective tools to control the West Nile vector <i>Culex pipiens</i>. Furthermore, the combined effect of thymol-carvacrol mixtures and their possible interactions were assessed. <i>Methods</i>: For determining larvicidal and ovicidal 50% lethal concentration (LC<sub>50</sub>), each monoterpenoid was tested at different concentrations (5&#8211;500 mg/L). Then, the fixed ratio method was used for evaluating their combinational efficacy. <i>Results</i>: Carvacrol was more toxic against larvae of <i>Cx. pipiens,</i> with a LC<sub>50</sub> value of 14 mg/L, whereas thymol exhibited a LC<sub>50</sub> value of 49 mg/L. Comparable trends of efficacy were observed when toxicity on <i>Cx. pipiens</i> eggs was investigated, with LC<sub>50</sub> values of 7 and 13 mg/L for carvacrol and thymol, respectively. In combinational toxicity assays, the mixture thymol-carvacrol at 1:4 ratio achieved a synergistic effect against larvae of <i>Cx. pipiens,</i> whereas an additive effect was observed on eggs. Other ratios showed antagonistic effects. <i>Conclusions</i>: Overall, our findings pointed out that the 1:4 ratio of thymol-carvacrol blend can enhance the insecticidal efficacy on <i>Cx. pipiens</i> young instars and can be considered further as active ingredient for developing botanical insecticides to be used in mosquito control operations.https://www.mdpi.com/1420-3049/24/10/1867biopesticideessential oilovicidal activitylarvicidal activitymosquito vector
collection DOAJ
language English
format Article
sources DOAJ
author Mohammad Reza Youssefi
Mohaddeseh Abouhosseini Tabari
Aryan Esfandiari
Sohrab Kazemi
Ali Akbar Moghadamnia
Stefania Sut
Stefano Dall’Acqua
Giovanni Benelli
Filippo Maggi
spellingShingle Mohammad Reza Youssefi
Mohaddeseh Abouhosseini Tabari
Aryan Esfandiari
Sohrab Kazemi
Ali Akbar Moghadamnia
Stefania Sut
Stefano Dall’Acqua
Giovanni Benelli
Filippo Maggi
Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>
Molecules
biopesticide
essential oil
ovicidal activity
larvicidal activity
mosquito vector
author_facet Mohammad Reza Youssefi
Mohaddeseh Abouhosseini Tabari
Aryan Esfandiari
Sohrab Kazemi
Ali Akbar Moghadamnia
Stefania Sut
Stefano Dall’Acqua
Giovanni Benelli
Filippo Maggi
author_sort Mohammad Reza Youssefi
title Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>
title_short Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>
title_full Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>
title_fullStr Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>
title_full_unstemmed Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector <i>Culex pipiens</i>
title_sort efficacy of two monoterpenoids, carvacrol and thymol, and their combinations against eggs and larvae of the west nile vector <i>culex pipiens</i>
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2019-05-01
description <i>Background</i>: Insect vector control is facing the challenges of resistance development and environmental hazards caused by synthetic pesticides. This has led to a considerable market opportunity for botanical insecticides. In this scenario, our study investigated the potential of selected bioactive monoterpenoids, carvacrol and thymol, as safe and effective tools to control the West Nile vector <i>Culex pipiens</i>. Furthermore, the combined effect of thymol-carvacrol mixtures and their possible interactions were assessed. <i>Methods</i>: For determining larvicidal and ovicidal 50% lethal concentration (LC<sub>50</sub>), each monoterpenoid was tested at different concentrations (5&#8211;500 mg/L). Then, the fixed ratio method was used for evaluating their combinational efficacy. <i>Results</i>: Carvacrol was more toxic against larvae of <i>Cx. pipiens,</i> with a LC<sub>50</sub> value of 14 mg/L, whereas thymol exhibited a LC<sub>50</sub> value of 49 mg/L. Comparable trends of efficacy were observed when toxicity on <i>Cx. pipiens</i> eggs was investigated, with LC<sub>50</sub> values of 7 and 13 mg/L for carvacrol and thymol, respectively. In combinational toxicity assays, the mixture thymol-carvacrol at 1:4 ratio achieved a synergistic effect against larvae of <i>Cx. pipiens,</i> whereas an additive effect was observed on eggs. Other ratios showed antagonistic effects. <i>Conclusions</i>: Overall, our findings pointed out that the 1:4 ratio of thymol-carvacrol blend can enhance the insecticidal efficacy on <i>Cx. pipiens</i> young instars and can be considered further as active ingredient for developing botanical insecticides to be used in mosquito control operations.
topic biopesticide
essential oil
ovicidal activity
larvicidal activity
mosquito vector
url https://www.mdpi.com/1420-3049/24/10/1867
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