Evaluation of differential serum expression of three factors and pulmonary function in patients with silicosis
Objectives Silicosis is a chronic occupational lung disease. As was previously found by the authors, some proteins increased in the lung tissue of activated rats, and protein tyrosine phosphatase non-receptor type 2 (PTPN2), factor B, and vaccinia-related kinase 1 (VRK1) showed highly differential e...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nofer Institute of Occupational Medicine
2021-08-01
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| Series: | International Journal of Occupational Medicine and Environmental Health |
| Subjects: | |
| Online Access: | http://ijomeh.eu/Evaluation-of-differential-serum-expression-of-three-factors-and-pulmonary-function,130485,0,2.html |
| Summary: | Objectives
Silicosis is a chronic occupational lung disease. As was previously found by the authors, some proteins increased in the lung tissue of
activated rats, and protein tyrosine phosphatase non-receptor type 2 (PTPN2), factor B, and vaccinia-related kinase 1 (VRK1) showed highly differential
expressions.
Material and Methods
In this study, serum and bronchoalveolar lavage fluid samples were collected from patients with silicosis
and healthy people to verify the expression of PTPN2, factor B, and VRK1. The diagnostic value of differentially expressed proteins for silicosis
was judged.
Results
The expression levels of serum PTPN2, VRK1, and factor B in patients with silicosis were significantly higher than those in
the control group (p < 0.01). Higher serum PTPN2 and factor B concentrations significantly and negatively correlated with the ratio of forced expiratory
volume in 1 s to forced vital capacity (FEV 1 /FVC), maximum vital capacity (VC max ), FEV 1 , and FVC, suggesting that the high expression
of PTPN2 and factor B is associated with decreased pulmonary ventilation function and restrictive ventilatory impairment in patients with silicosis.
All area under curve (AUC) measurements generated from single detection events were >0.744, with PTPN2 reaching the highest value (0.858).
The AUC, sensitivity, and specificity for the combined diagnosis using factor B and PTPN2 were 0.907, 86.91% and 85.07%, respectively, for factor B
and PTPN2. The 3 differentially expressed proteins are potential classes of predictive biomarkers for silicosis.
Conclusions
Regarding the economy
and test practicality, the best diagnostic combination is factor B and PTPN2 for the analysis of AUC, sensitivity and specificity. Int J Occup Med
Environ Health. 2021;34(4):527–40 |
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| ISSN: | 1232-1087 1896-494X |