Effect of alternating the magnetic feld on phosphate metabolism in the nervous system of Helix pomatia

• Main Authors: Ljiljana M Nikolic, Milos B Rokic, Natasa V Todorovic, Gordana S Kartelija, Miodrag S Nedeljkovic, Joanna S Zakrzewska
• Format: Article
• Language: English
• Published: BMC 2010-01-01
• Series: Biological Research
• Subjects: ELF-MF; garden snail; 31P NMR spectroscopy; phosphate turnover enzymes
• Online Access: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200012
• ISSN: 0716-9760; 0717-6287

The effect of extremely low frequency magnetic felds (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The infuence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by 31P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na+/K+-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.