Biosynthesis of phosphatidyl glycerophosphate in Escherichia coli

• Main Authors: Ying-Ying Chang, Eugene P. Kennedy
• Format: Article
• Language: English
• Published: Elsevier 1967-09-01
• Series: Journal of Lipid Research
• Subjects: phosphatidyl; glycerophosphate; CDP-diglyceride; l-glycerol 3-phosphate; enzymatic; biosynthesis
• Online Access: http://www.sciencedirect.com/science/article/pii/S002222752038901X

An enzyme (l-glycerol 3-phosphate: CMP phosphatidyltransferase) catalyzing the synthesis of phosphatidyl glycerophosphate from CDP-diglyceride and l-glycerol 3-phosphate has been rendered soluble by treatment of the particulate, membrane-containing fraction of E. coli with Triton X-100 and has been partially purified. The enzyme, devoid of phosphatidyl glycerophosphatase activity, is specific for l-glycerol 3-phosphate and is completely dependent upon added Mg++ or Mn++ for activity. It has high affinity for CDP-diglyceride and can be used for the assay of this nucleotide.Other properties of the enzyme are also described.