MORPHOGENETICAL AND HISTOLOGICAL STUDIES OF „IN VITRO” ANTHER CULTURES OF BRASSICA OLERACEA L.

• Main Authors: Daniela Nicuta, Irina Toma, Gogu Gheorghita
• Format: Article
• Language: English
• Published: "Alexandru Ioan Cuza" University of Iași 2005-08-01
• Series: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
• Online Access: http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/287

The anthers were inoculated on 8 hormonic variants of MS (Murashige-Skoog, 1962) medium and on one variant of B5 (Gamborg, 1968) slightly changed medium formula. The main morphogenetic reaction was callus formation. The callus’s consistency and colour differed depending on the hormonic variant. The friable callus for all the tested genotypes had caulogenetic capacity. Its passage on differentiation media comprising cytokinins led to new shoots formation, the intensity of this phenomenon varied with the genotype and with the initial medium that provided the respective callus. The histological studies of calluses or of anther shoots pointed out callus structure and the numerous histogenetic stages due to growth regulators, as well as in vitro regenerated shoots’ structure.