An Original HPLC Method with Coulometric Detection to Monitor Hydroxyl Radical Generation via Fenton Chemistry

An Original HPLC Method with Coulometric Detection to Monitor Hydroxyl Radical Generation via Fenton Chemistry

Hydroxyl radicals (•OH) can be generated via Fenton chemistry catalyzed by transition metals. An in vitro Fenton system was developed to test both the inhibition and stimulation of •OH formation, by monitoring salicylate aromatic hydroxylation derivatives as markers of &#8226...

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Bibliographic Details
Main Authors: Maria Carmen Catapano, Michele Protti, Tommaso Fontana, Roberto Mandrioli, Přemysl Mladěnka, Laura Mercolini
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Molecules
Subjects:
antioxidant
Fenton’s reaction
hydroxyl radical
HPLC-ED
coulometric detector
method validation
Online Access:https://www.mdpi.com/1420-3049/24/17/3066

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