Morphometric Approach to Pulp Fibroblast Development in Tooth Germ

This paper builds a morphometric framework for the analysis of dental pulp fibroblast evolution during tooth development. We investigated 15 tooth germs (cases) organized, by histological criteria, in three groups corresponding to cap, early bell, and late bell stages, respectively. Each group compr...

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Main Authors: Irina-Draga Căruntu, Sergiu Daniel Săvinescu, Cornelia Amălinei
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2014/836583
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spelling doaj-dcb83893ae424e90906fb83eaa9869252020-11-24T20:55:08ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/836583836583Morphometric Approach to Pulp Fibroblast Development in Tooth GermIrina-Draga Căruntu0Sergiu Daniel Săvinescu1Cornelia Amălinei2Department of Morpho-Functional Sciences—Histology, Grigore T. Popa University of Medicine and Pharmacy of Iaşi, 16 University Street, 700115 Iaşi, RomaniaDepartment of Morpho-Functional Sciences—Histology, Grigore T. Popa University of Medicine and Pharmacy of Iaşi, 16 University Street, 700115 Iaşi, RomaniaDepartment of Morpho-Functional Sciences—Histology, Grigore T. Popa University of Medicine and Pharmacy of Iaşi, 16 University Street, 700115 Iaşi, RomaniaThis paper builds a morphometric framework for the analysis of dental pulp fibroblast evolution during tooth development. We investigated 15 tooth germs (cases) organized, by histological criteria, in three groups corresponding to cap, early bell, and late bell stages, respectively. Each group comprised five cases. The morphometric description used the following parameters: area A, perimeter P—automatically extracted by a color segmentation technique, and form factor (FF)—calculated as 4πA/P2. The designed framework operated at inter- and intragroup levels. The intergroup analysis quantified the differences between groups, in the sense of a relative distance (RD) adequately defined by mean-value scaling. We showed that the stage of early bell is approximately 5 times closer to late bell than to cap. The quantification procedure required concomitant information about A, P parameters (as P versus A dependences, or FF values), whereas the procedure failed for A or P separately used. The intragroup analysis quantified the similarity of the cases belonging to the same stage. We proved that, unlike the intergroup tests, the individual exploitation of all three descriptors A, P, and FF is effective, yielding highly compatible results. Within any group, most cases presented RDs less than 10% from the group mean value, regardless of the descriptor type.http://dx.doi.org/10.1155/2014/836583
collection DOAJ
language English
format Article
sources DOAJ
author Irina-Draga Căruntu
Sergiu Daniel Săvinescu
Cornelia Amălinei
spellingShingle Irina-Draga Căruntu
Sergiu Daniel Săvinescu
Cornelia Amălinei
Morphometric Approach to Pulp Fibroblast Development in Tooth Germ
BioMed Research International
author_facet Irina-Draga Căruntu
Sergiu Daniel Săvinescu
Cornelia Amălinei
author_sort Irina-Draga Căruntu
title Morphometric Approach to Pulp Fibroblast Development in Tooth Germ
title_short Morphometric Approach to Pulp Fibroblast Development in Tooth Germ
title_full Morphometric Approach to Pulp Fibroblast Development in Tooth Germ
title_fullStr Morphometric Approach to Pulp Fibroblast Development in Tooth Germ
title_full_unstemmed Morphometric Approach to Pulp Fibroblast Development in Tooth Germ
title_sort morphometric approach to pulp fibroblast development in tooth germ
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2014-01-01
description This paper builds a morphometric framework for the analysis of dental pulp fibroblast evolution during tooth development. We investigated 15 tooth germs (cases) organized, by histological criteria, in three groups corresponding to cap, early bell, and late bell stages, respectively. Each group comprised five cases. The morphometric description used the following parameters: area A, perimeter P—automatically extracted by a color segmentation technique, and form factor (FF)—calculated as 4πA/P2. The designed framework operated at inter- and intragroup levels. The intergroup analysis quantified the differences between groups, in the sense of a relative distance (RD) adequately defined by mean-value scaling. We showed that the stage of early bell is approximately 5 times closer to late bell than to cap. The quantification procedure required concomitant information about A, P parameters (as P versus A dependences, or FF values), whereas the procedure failed for A or P separately used. The intragroup analysis quantified the similarity of the cases belonging to the same stage. We proved that, unlike the intergroup tests, the individual exploitation of all three descriptors A, P, and FF is effective, yielding highly compatible results. Within any group, most cases presented RDs less than 10% from the group mean value, regardless of the descriptor type.
url http://dx.doi.org/10.1155/2014/836583
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