Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm

Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm

Single Nucelotide Polymorphisms (SNPs) have become the most widely used markers in many current genetic applications. Here we report the discovery of nine new SNPs in olives by direct partial sequencing of two genes (OEX and OEW) in sixteen Tunisian cultivars. The SNP markers were then used to genot...

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Main Authors: Imen Rekik Hakim, Naziha Grati Kammoun, Emna Makhloufi, Ahmed Rebaï
Format: Article
Language:English
Published: MDPI AG 2009-12-01
Series:Diversity
Subjects:
cultivar genotyping
genetic diversity
Olea europaea L.
SNP markers
Online Access:http://www.mdpi.com/1424-2818/2/1/17/
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spelling doaj-dca201a4723a4f29b9d0aee042c672af2020-11-24T21:02:01ZengMDPI AGDiversity1424-28182009-12-0121172710.3390/d2010017Discovery and Potential of SNP Markers in Characterization of Tunisian Olive GermplasmImen Rekik HakimNaziha Grati KammounEmna MakhloufiAhmed RebaïSingle Nucelotide Polymorphisms (SNPs) have become the most widely used markers in many current genetic applications. Here we report the discovery of nine new SNPs in olives by direct partial sequencing of two genes (OEX and OEW) in sixteen Tunisian cultivars. The SNP markers were then used to genotype 24 olive cultivars and assess the level of genetic diversity. Power of discrimination of SNP markers was then compared to that of microsatellites (SSRs). A combination of SSR and SNP markers was finally proposed that can be used for cultivars identification in juvenile step or for oil traceability. http://www.mdpi.com/1424-2818/2/1/17/cultivar genotypinggenetic diversityOlea europaea L.SNP markers
collection DOAJ
language English
format Article
sources DOAJ
author Imen Rekik Hakim
Naziha Grati Kammoun
Emna Makhloufi
Ahmed Rebaï
spellingShingle Imen Rekik Hakim
Naziha Grati Kammoun
Emna Makhloufi
Ahmed Rebaï
Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm
Diversity
cultivar genotyping
genetic diversity
Olea europaea L.
SNP markers
author_facet Imen Rekik Hakim
Naziha Grati Kammoun
Emna Makhloufi
Ahmed Rebaï
author_sort Imen Rekik Hakim
title Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm
title_short Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm
title_full Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm
title_fullStr Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm
title_full_unstemmed Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm
title_sort discovery and potential of snp markers in characterization of tunisian olive germplasm
publisher MDPI AG
series Diversity
issn 1424-2818
publishDate 2009-12-01
description Single Nucelotide Polymorphisms (SNPs) have become the most widely used markers in many current genetic applications. Here we report the discovery of nine new SNPs in olives by direct partial sequencing of two genes (OEX and OEW) in sixteen Tunisian cultivars. The SNP markers were then used to genotype 24 olive cultivars and assess the level of genetic diversity. Power of discrimination of SNP markers was then compared to that of microsatellites (SSRs). A combination of SSR and SNP markers was finally proposed that can be used for cultivars identification in juvenile step or for oil traceability.
topic cultivar genotyping
genetic diversity
Olea europaea L.
SNP markers
url http://www.mdpi.com/1424-2818/2/1/17/
work_keys_str_mv AT imenrekikhakim discoveryandpotentialofsnpmarkersincharacterizationoftunisianolivegermplasm
AT nazihagratikammoun discoveryandpotentialofsnpmarkersincharacterizationoftunisianolivegermplasm
AT emnamakhloufi discoveryandpotentialofsnpmarkersincharacterizationoftunisianolivegermplasm
AT ahmedrebai discoveryandpotentialofsnpmarkersincharacterizationoftunisianolivegermplasm
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