Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ

The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the prote...

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Main Authors:	Duri Rungger, Lisbeth Muster, Oleg Georgiev, Elisabeth Rungger-Brändle
Format:	Article
Language:	English
Published:	The Company of Biologists 2017-02-01
Series:	Biology Open
Subjects:	Gene delivery Homologous recombination Oogenesis Vitellogenin pathway
Online Access:	http://bio.biologists.org/content/6/2/290

id	doaj-dc8c28c752854db59a2e843f5773109c
record_format	Article
spelling	doaj-dc8c28c752854db59a2e843f5773109c2021-06-02T13:17:14ZengThe Company of BiologistsBiology Open2046-63902017-02-016229029510.1242/bio.022376022376Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situDuri Rungger0Lisbeth Muster1Oleg Georgiev2Elisabeth Rungger-Brändle3 Station de Zoologie expérimentale, Department of Genetics and Evolution, University of Geneva, 154 route de Malagnou, Chêne-Bougeries 1224, Switzerland Station de Zoologie expérimentale, Department of Genetics and Evolution, University of Geneva, 154 route de Malagnou, Chêne-Bougeries 1224, Switzerland Institute of Molecular Life Sciences, University of Zurich-Irchel, Winterthurer Strasse 190, Zurich 8057, Switzerland Biologie cellulaire, University Eye Clinic, 20 rue Alcide Jentzer, Geneva 4 1211, Switzerland The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. Functioning of the shuttle protein is documented by transporting DNA molecules that, upon intramolecular homologous recombination within the oocyte nucleus, express easily traceable markers such as green fluorescence or tetracycline resistance.http://bio.biologists.org/content/6/2/290Gene deliveryHomologous recombinationOogenesisVitellogenin pathway
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Duri Rungger Lisbeth Muster Oleg Georgiev Elisabeth Rungger-Brändle
spellingShingle	Duri Rungger Lisbeth Muster Oleg Georgiev Elisabeth Rungger-Brändle Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ Biology Open Gene delivery Homologous recombination Oogenesis Vitellogenin pathway
author_facet	Duri Rungger Lisbeth Muster Oleg Georgiev Elisabeth Rungger-Brändle
author_sort	Duri Rungger
title	Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ
title_short	Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ
title_full	Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ
title_fullStr	Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ
title_full_unstemmed	Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ
title_sort	oocyte shuttle, a recombinant protein transporting donor dna into the xenopus oocyte in situ
publisher	The Company of Biologists
series	Biology Open
issn	2046-6390
publishDate	2017-02-01
description	The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. Functioning of the shuttle protein is documented by transporting DNA molecules that, upon intramolecular homologous recombination within the oocyte nucleus, express easily traceable markers such as green fluorescence or tetracycline resistance.
topic	Gene delivery Homologous recombination Oogenesis Vitellogenin pathway
url	http://bio.biologists.org/content/6/2/290
work_keys_str_mv	AT durirungger oocyteshuttlearecombinantproteintransportingdonordnaintothexenopusoocyteinsitu AT lisbethmuster oocyteshuttlearecombinantproteintransportingdonordnaintothexenopusoocyteinsitu AT oleggeorgiev oocyteshuttlearecombinantproteintransportingdonordnaintothexenopusoocyteinsitu AT elisabethrunggerbrandle oocyteshuttlearecombinantproteintransportingdonordnaintothexenopusoocyteinsitu
_version_	1721404126447796224

Oocyte shuttle, a recombinant protein transporting donor DNA into the Xenopus oocyte in situ

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