Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
Abstract High-protein diets often lead to an increase in urea concentration in follicular fluid of dairy cows, which may reduce oocyte competence. In the present study, maturation media were supplemented with urea (0, 20, 40 mg/dl), and amino acids (AAs) turnover was evaluated in the 24-h spent medi...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-08-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-018-30774-2 |