Single tube, high throughput cloning of inverted repeat constructs for double-stranded RNA expression.

Single tube, high throughput cloning of inverted repeat constructs for double-stranded RNA expression.

BACKGROUND: RNA interference (RNAi) has emerged as a powerful tool for the targeted knockout of genes for functional genomics, system biology studies and drug discovery applications. To meet the requirements for high throughput screening in plants we have developed a new method for the rapid assembl...

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Bibliographic Details
Main Authors: Brian Hauge, Christopher Oggero, Nicole Nguyen, Changlin Fu, Fenggao Dong
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2745663?pdf=render

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