| Summary: | The purpose of this study was to determine the stocks of available P and S, total N, and oxidizable C at depth in an Oxisol cultivated with Eucalyptus in Brazil following different timber harvest intensities and fertilizer application over 12 years. The harvest regimes considered were (i) conventional stem-only harvest (all forest residues were maintained on the soil); (ii) whole-tree harvest (only litter was maintained on the soil—all slash, stemwood, and bark were removed); and (iii) whole-tree harvest + litter layer removal. The site was planted in 2004 considering three timber harvest intensities, some with and some without N and P fertilization. In 2012 the experiment was reinstalled, and all the treatments were reapplied in the each plot. From 2004 to 2016, nutrient accumulation and soil N, P, and S stocks were assessed in the 0–20 cm layer. Also in 2016, soil N, P, S, and oxidizable C stocks were measured to 2 m depth. For each treatment, the net balance of N, P, and S were calculated from soil stocks and harvest outputs during two forest rotations. A reduction in all nutrient stocks was observed in the 0–20 cm layer for all treatments. For N, this reduction was 20% smaller in the stem-only harvest treatment and 40% higher when no N fertilizer was applied, when compared to other treatments. Stem-only harvest treatment was observed to reduce the loss of N, P, and S due to harvest by 300, 30, and 25 kg·ha−1, respectively, when compared to the whole-tree harvest + litter layer removal treatment.
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