Investigation of synergistic effect of cuo nanoparticles and nisin on genomic of Escherichia coli bacteria

• Main Authors: Trifa Sheikhaghaiy, Bahram Golestani Eimani
• Format: Article
• Language: English
• Published: Iranian Environmental Mutagen Society 2018-10-01
• Series: Journal of Water and Environmental Nanotechnology
• Subjects: copper oxide nanoparticles; nisin; escherichia coli; synergistic; rapd-pcr
• Online Access: http://www.jwent.net/article_33352_6720645b83d9393503e2658877656c4b.pdf

Given the gradual development of drug resistance in different bacterial species, it is necessary to search for new drugs with effective broad spectrum antimicrobial activity. Therefore, recent studies on various nanometal oxides such as copper oxide and on antibacterial peptides including nisin as antibacterial agents are especially important. The present study aimed to investigate the synergistic effect of nisin-conjugated copper oxide nanoparticles (CuO NPs) on the genome of E. coli selected as a Gram-negative model. After being cultured in a Nutrient Broth medium, the bacteria were treated with CuO NPs at 15, 30, 40, and 60μg/mL, with nisin at 30, 60, 90, and 120μg/mL, and with nisin-conjugated CuO NPs at 10, 20, and 30μg/mL and were then incubated. The optical densities of the samples were read at 600nm and their DNA was extracted. RAPD-PCR was used to study genomic effects, and statistical analysis was performed employing NTSYS-PC based on the DICE coefficient, the similarity matrix, and the drawn dendrogram. Results showed that the combination of CuO NPs and nisin had synergistic effects and was able to inhibit growth more than either of them used alone. However, this combination had no synergistic effects on the genome and caused minimal changes in the DNA sequence.