Determinação da concentração de paclobutrazol por cromatografia líquida de alta eficiência e espectroscopia Determination of paclobutrazol concentration by high performance liquid chromatography and spectroscopy

• Main Authors: Fernanda Leitão Vaz, Mônica Lúcia Barros Milfont, Ana Maria Souto-Maior, Ester Ribeiro Gouveia
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2007-04-01
• Series: Química Nova
• Subjects: paclobutrazol; HPLC; spectroscopy
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000200007

<abstract language="eng">Paclobutrazol is a plant growth retardant which is used world-wide for increasing the yield of cereal crops. However, this compound remains active in the soil for several years and can severely affect the growth and development of subsequent crops, mainly by reducing vegetative vigor. The aim of this work was to develop and validate methods for the determination of paclobutrazol concentrations by both high performance liquid chromatography and spectroscopy. Both methods were satisfactory and showed appropriately low quantification limits. The determination by spectroscopy has, however, the advantage of being a method significantly less expensive than high performance liquid chromatography.