TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).

TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).

GTPase of the immune associated nucleotide binding protein (GIMAP) family of proteins are expressed essentially in cells of the hematopoietic system. Mutation in the founding member of this gene family, Gimap5, results in the lymphopenic phenotype in Bio-Breeding diabetes prone rats. In mice, deleti...

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Main Authors: Xi-Lin Chen, Daniel Serrano, Farnaz Ghobadi, Marian Mayhue, Kasper Hoebe, Subburaj Ilangumaran, Sheela Ramanathan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0151837
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spelling doaj-dc3ce9249b754dedaae5216bd7312f992021-03-03T19:46:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015183710.1371/journal.pone.0151837TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).Xi-Lin ChenDaniel SerranoFarnaz GhobadiMarian MayhueKasper HoebeSubburaj IlangumaranSheela RamanathanGTPase of the immune associated nucleotide binding protein (GIMAP) family of proteins are expressed essentially in cells of the hematopoietic system. Mutation in the founding member of this gene family, Gimap5, results in the lymphopenic phenotype in Bio-Breeding diabetes prone rats. In mice, deletion of functional Gimap5 gene affects the survival and renewal of hematopoietic stem cells in addition to the defects observed in T cells. Here we show that T cells from OTII TCR-transgenic Gimap5sph/sph mice do not proliferate in response to its cognate antigen. Furthermore, T cells from Gimap5 mutant rats and mice show decreased phosphorylation of STAT5 following stimulation with IL-7. Our results suggest that functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells.https://doi.org/10.1371/journal.pone.0151837
collection DOAJ
language English
format Article
sources DOAJ
author Xi-Lin Chen
Daniel Serrano
Farnaz Ghobadi
Marian Mayhue
Kasper Hoebe
Subburaj Ilangumaran
Sheela Ramanathan
spellingShingle Xi-Lin Chen
Daniel Serrano
Farnaz Ghobadi
Marian Mayhue
Kasper Hoebe
Subburaj Ilangumaran
Sheela Ramanathan
TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).
PLoS ONE
author_facet Xi-Lin Chen
Daniel Serrano
Farnaz Ghobadi
Marian Mayhue
Kasper Hoebe
Subburaj Ilangumaran
Sheela Ramanathan
author_sort Xi-Lin Chen
title TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).
title_short TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).
title_full TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).
title_fullStr TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).
title_full_unstemmed TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5).
title_sort tcr and il-7 signaling are altered in the absence of functional gtpase of the immune associated nucleotide binding protein 5 (gimap5).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2016-01-01
description GTPase of the immune associated nucleotide binding protein (GIMAP) family of proteins are expressed essentially in cells of the hematopoietic system. Mutation in the founding member of this gene family, Gimap5, results in the lymphopenic phenotype in Bio-Breeding diabetes prone rats. In mice, deletion of functional Gimap5 gene affects the survival and renewal of hematopoietic stem cells in addition to the defects observed in T cells. Here we show that T cells from OTII TCR-transgenic Gimap5sph/sph mice do not proliferate in response to its cognate antigen. Furthermore, T cells from Gimap5 mutant rats and mice show decreased phosphorylation of STAT5 following stimulation with IL-7. Our results suggest that functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells.
url https://doi.org/10.1371/journal.pone.0151837
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