| Summary: | Chih-Chien Hu,1– 4 Chih-Hsiang Chang,1– 3 Yuhan Chang,1– 3 Jang-Hsing Hsieh,5,6 Steve Wen-Neng Ueng1– 3 1Bone and Joint Research Center, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 2Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 3College of Medicine, Chang Gung University, Kweishan, Taoyuan 33302, Taiwan; 4Department of Orthopedic Surgery, Xiamen Chang Gung Hospital, Xiamen, Fujian, People’s Republic of China; 5Department of Materials Engineering, Ming Chi University of Technology, Taishan, Taipei 24301, Taiwan; 6Center for Thin Film Technologies and Applications, Ming Chi University of Technology, Taishan, Taipei 24301, TaiwanCorrespondence: Steve Wen-Neng UengDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, No. 5, Fu-Hsin St, Kweishan, Taoyuan 33305, TaiwanTel +88633281200-2420Fax +886 33278113Email wenneng@adm.cgmh.org.twPurpose: In this study, a novel oxygenated nanocomposite thin film, TaON-Ag, was investigated in vitro and in vivo to evaluate its biocompatibility and antibacterial ability.Material and Methods: The antibacterial ability of TaON-Ag nanocomposite-coated titanium (Ti) was evaluated using the Kirby-Bauer disk diffusion susceptibility test. The effects of TaON-Ag nanocomposite-coated metal on osteogenesis were further evaluated in an in vitro osteogenic culture model with rat marrow-derived mesenchymal stem cells (rMSCs). Furthermore, titanium rods coated with TaON-Ag were implanted into a rat femur fracture model either with or without osteomyelitis to investigate the effects of TaON-Ag in osteogenesis.Results: The TaON-Ag-coated Ti exhibited an effective antibacterial effect against Staphylococcus aureus, coagulase-negative Staphylococcus, and the Gram-negative strains Escherichia coli and Pseudomonas aeruginosa. Using an osteogenic culture with rMSCs and a rat femoral fracture model, the TaON-Ag-coated Ti did not interfere with the ossification of rMSCs in vitro or during fracture healing in vivo. Field-emission scanning electron microscopy (FE-SEM) revealed that coating with TaON-Ag could inhibit pathogen adhesion and biofilm formation in both Staphylococcus aureus and Escherichia coli.Conclusion: Using the proposed novel oxygenation process, TaON-Ag nanocomposite-coated Ti yielded robust biocompatibility and antibacterial ability against common microorganisms in orthopedic infections, thereby demonstrating potential for use in clinical applications.Keywords: silver nanoparticles, ossification, antibacterial ability, biofilm
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