Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development
Cold-induced mortality has historically been a key aspect of mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. We used RNA-seq analysis to monitor gene expression pat...
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2016-07-01
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| Online Access: | https://peerj.com/articles/2109.pdf |
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doaj-dc23b519ddc6459eabbb0d94658414802020-11-24T23:14:31ZengPeerJ Inc.PeerJ2167-83592016-07-014e210910.7717/peerj.2109Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring developmentJeanne A. Robert0Tiffany Bonnett1Caitlin Pitt2Luke J. Spooner3Jordie Fraser4Macaire M.S. Yuen5Christopher I. Keeling6Jörg Bohlmann7Dezene P.W. Huber8Department of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, CanadaDepartment of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, CanadaDepartment of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, CanadaDepartment of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, CanadaDepartment of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, CanadaDepartment of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, CanadaCold-induced mortality has historically been a key aspect of mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. We used RNA-seq analysis to monitor gene expression patterns of mountain pine beetle larvae at four time points during their overwintering period—early-autumn, late-autumn, early-spring, and late-spring. Changing transcript profiles over the winter indicates a multipronged physiological response from larvae that is broadly characterized by gene transcripts involved in insect immune responses and detoxification during the autumn. In the spring, although transcripts associated with developmental process are present, there was no particular biological process dominating the transcriptome.https://peerj.com/articles/2109.pdfCold toleranceMountain pine beetleInsect overwinteringGlycerolPhysiological stressLodgepole pine |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jeanne A. Robert Tiffany Bonnett Caitlin Pitt Luke J. Spooner Jordie Fraser Macaire M.S. Yuen Christopher I. Keeling Jörg Bohlmann Dezene P.W. Huber |
| spellingShingle |
Jeanne A. Robert Tiffany Bonnett Caitlin Pitt Luke J. Spooner Jordie Fraser Macaire M.S. Yuen Christopher I. Keeling Jörg Bohlmann Dezene P.W. Huber Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development PeerJ Cold tolerance Mountain pine beetle Insect overwintering Glycerol Physiological stress Lodgepole pine |
| author_facet |
Jeanne A. Robert Tiffany Bonnett Caitlin Pitt Luke J. Spooner Jordie Fraser Macaire M.S. Yuen Christopher I. Keeling Jörg Bohlmann Dezene P.W. Huber |
| author_sort |
Jeanne A. Robert |
| title |
Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development |
| title_short |
Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development |
| title_full |
Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development |
| title_fullStr |
Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development |
| title_full_unstemmed |
Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development |
| title_sort |
gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development |
| publisher |
PeerJ Inc. |
| series |
PeerJ |
| issn |
2167-8359 |
| publishDate |
2016-07-01 |
| description |
Cold-induced mortality has historically been a key aspect of mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. We used RNA-seq analysis to monitor gene expression patterns of mountain pine beetle larvae at four time points during their overwintering period—early-autumn, late-autumn, early-spring, and late-spring. Changing transcript profiles over the winter indicates a multipronged physiological response from larvae that is broadly characterized by gene transcripts involved in insect immune responses and detoxification during the autumn. In the spring, although transcripts associated with developmental process are present, there was no particular biological process dominating the transcriptome. |
| topic |
Cold tolerance Mountain pine beetle Insect overwintering Glycerol Physiological stress Lodgepole pine |
| url |
https://peerj.com/articles/2109.pdf |
| work_keys_str_mv |
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