Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities

In this study, an attempt was made to synthesize silver nanoparticles (Ag-NPs) using Grewia optiva leaf extract and isolated compounds. The bioreductant capacity of Grewia optiva leaf extract for the synthesis of Ag-NPs was assessed using various confirmatory techniques like thermogravimetric analys...

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Main Authors: Muhammad Iftikhar, Muhammad Zahoor, Sumaira Naz, Nausheen Nazir, Gaber El-Saber Batiha, Riaz Ullah, Ahmed Bari, Muhammad Hanif, Hafiz Majid Mahmood
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Journal of Nanomaterials
Online Access:http://dx.doi.org/10.1155/2020/8949674
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spelling doaj-dbf16bda3032431b9bb1ce1e983d136d2020-11-25T03:41:15ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292020-01-01202010.1155/2020/89496748949674Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological ActivitiesMuhammad Iftikhar0Muhammad Zahoor1Sumaira Naz2Nausheen Nazir3Gaber El-Saber Batiha4Riaz Ullah5Ahmed Bari6Muhammad Hanif7Hafiz Majid Mahmood8Department of Biochemistry, University of Malakand, Chakdara Dir Lower Khyber Pakhtunkhwa, PakistanDepartment of Biochemistry, University of Malakand, Chakdara Dir Lower Khyber Pakhtunkhwa, PakistanDepartment of Biochemistry, University of Malakand, Chakdara Dir Lower Khyber Pakhtunkhwa, PakistanDepartment of Biochemistry, University of Malakand, Chakdara Dir Lower Khyber Pakhtunkhwa, PakistanDepartment of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Al Beheira, EgyptMedicinal, Aromatic and Poisonous Plants Research Centre (MAPRC), College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaCentral Laboratory College of Pharmacy King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaRiphah Institute of Pharmaceutical Sciences, Islamabad, PakistanDepartment of Pharmacology, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaIn this study, an attempt was made to synthesize silver nanoparticles (Ag-NPs) using Grewia optiva leaf extract and isolated compounds. The bioreductant capacity of Grewia optiva leaf extract for the synthesis of Ag-NPs was assessed using various confirmatory techniques like thermogravimetric analysis (TGA), particle size analysis (PSA), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and UV-Visible spectroscopy. The presence of various bioactive compounds in leaf aqueous extract was confirmed through HPLC analysis, and 8 compounds were identified among the different peaks present in the chromatogram. Biopotencies like antioxidant, antibacterial, and effect on hair growth were determined for extract and NPs. Antioxidant capacities were assessed through standard ABTS and DPPH methods. The antibacterial potential was evaluated in terms of zone of inhibition, minimum bactericidal concentration, and minimum inhibitory concentration of the Ag-NPs and the leaf extract against selected strains of bacteria, whereas the effect on growth of rabbit hair was studied through topical treatment for a specific period of time. Better antibacterial and DPPH and ABTS free radical inhibition was observed for the formulated Ag-NPs as compared to leaf extract. The previously isolated eight compounds from this plant’s chloroform and ethyl acetate extracts were also tested for their bioreductant capacities. Out of them, the highest amount of precipitates was obtained with compound VII ((2,5-dihydroxyphenyl)-3′,6′,8′-trihydroxyl-4H-chromen-4′-one). The study implies that the biogenically engineered nanoscale particles could have promising biological activities in comparison to parental extract and they need to be investigated further as potential therapeutic agents to be used as antibacterial and antioxidant agents and for hair growth enhancement.http://dx.doi.org/10.1155/2020/8949674
collection DOAJ
language English
format Article
sources DOAJ
author Muhammad Iftikhar
Muhammad Zahoor
Sumaira Naz
Nausheen Nazir
Gaber El-Saber Batiha
Riaz Ullah
Ahmed Bari
Muhammad Hanif
Hafiz Majid Mahmood
spellingShingle Muhammad Iftikhar
Muhammad Zahoor
Sumaira Naz
Nausheen Nazir
Gaber El-Saber Batiha
Riaz Ullah
Ahmed Bari
Muhammad Hanif
Hafiz Majid Mahmood
Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities
Journal of Nanomaterials
author_facet Muhammad Iftikhar
Muhammad Zahoor
Sumaira Naz
Nausheen Nazir
Gaber El-Saber Batiha
Riaz Ullah
Ahmed Bari
Muhammad Hanif
Hafiz Majid Mahmood
author_sort Muhammad Iftikhar
title Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities
title_short Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities
title_full Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities
title_fullStr Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities
title_full_unstemmed Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities
title_sort green synthesis of silver nanoparticles using grewia optiva leaf aqueous extract and isolated compounds as reducing agent and their biological activities
publisher Hindawi Limited
series Journal of Nanomaterials
issn 1687-4110
1687-4129
publishDate 2020-01-01
description In this study, an attempt was made to synthesize silver nanoparticles (Ag-NPs) using Grewia optiva leaf extract and isolated compounds. The bioreductant capacity of Grewia optiva leaf extract for the synthesis of Ag-NPs was assessed using various confirmatory techniques like thermogravimetric analysis (TGA), particle size analysis (PSA), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and UV-Visible spectroscopy. The presence of various bioactive compounds in leaf aqueous extract was confirmed through HPLC analysis, and 8 compounds were identified among the different peaks present in the chromatogram. Biopotencies like antioxidant, antibacterial, and effect on hair growth were determined for extract and NPs. Antioxidant capacities were assessed through standard ABTS and DPPH methods. The antibacterial potential was evaluated in terms of zone of inhibition, minimum bactericidal concentration, and minimum inhibitory concentration of the Ag-NPs and the leaf extract against selected strains of bacteria, whereas the effect on growth of rabbit hair was studied through topical treatment for a specific period of time. Better antibacterial and DPPH and ABTS free radical inhibition was observed for the formulated Ag-NPs as compared to leaf extract. The previously isolated eight compounds from this plant’s chloroform and ethyl acetate extracts were also tested for their bioreductant capacities. Out of them, the highest amount of precipitates was obtained with compound VII ((2,5-dihydroxyphenyl)-3′,6′,8′-trihydroxyl-4H-chromen-4′-one). The study implies that the biogenically engineered nanoscale particles could have promising biological activities in comparison to parental extract and they need to be investigated further as potential therapeutic agents to be used as antibacterial and antioxidant agents and for hair growth enhancement.
url http://dx.doi.org/10.1155/2020/8949674
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