Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.

Ceramide is a key compound in sphingolipid metabolism. Dynamics of ceramide synthesis is important in the several biological processes, such as induction of apoptosis or insulin resistance. So far, its de novo synthesis rate was evaluated indirectly, based on the content of the compound, its interme...

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Main Authors: Piotr Zabielski, Agnieszka Urszula Błachnio-Zabielska, Beata Wójcik, Adrian Chabowski, Jan Górski
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5667851?pdf=render
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spelling doaj-dbc3ae6c638e45439b8a3e973780038f2020-11-25T01:14:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018713610.1371/journal.pone.0187136Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.Piotr ZabielskiAgnieszka Urszula Błachnio-ZabielskaBeata WójcikAdrian ChabowskiJan GórskiCeramide is a key compound in sphingolipid metabolism. Dynamics of ceramide synthesis is important in the several biological processes, such as induction of apoptosis or insulin resistance. So far, its de novo synthesis rate was evaluated indirectly, based on the content of the compound, its intermediates and the activity of respective enzymes. The aim of the present study was to directly measure ceramide synthesis rate (FSR) in different muscle types under varied plasma FFA supply in rat with the use of [U-13C] palmitate tracer and LC/MS/MS. The experiments were carried out on male Wistar rats, divided into three groups: 1-control, 2-with elevated plasma free fatty acid (FFA) concentration by means of intralipid and heparin, 3-with reduced plasma FFA concentration by means of nicotinic acid. The stable plasma FFA concentration and plasma [U-13C] palmitate enrichment was maintained for two hours by simultaneous infusion of the tracer and the respective compounds. At the end of the experiment, samples of blood from the abdominal aorta, the heart, diaphragm, soleus and white section of the gastrocnemius were taken. Muscle sphinganine, sphingosine and ceramide content and enrichment and plasma palmitate enrichment was measured with the use of LC/MS/MS. Plasma FFA concentration and composition was measured by means of gas-liquid chromatography. Under basal conditions ceramide FSR in the heart and the diaphragm was higher than in the soleus and the white gastrocnemius. Elevation in the plasma FFA concentration increased the FSR and ceramide content in each muscle, which correlated with increased HOMA-IR. The highest FSR was noted in the heart. Reduction in the plasma FFA concentration decreased ceramide FSR in each muscle type, which was accompanied by marked reduction in HOMA-IR. It is concluded that ceramide FSR depends on both the muscle type and the plasma FFA supply and is correlated with whole body insulin sensitivity under varying plasma FFA supply.http://europepmc.org/articles/PMC5667851?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Piotr Zabielski
Agnieszka Urszula Błachnio-Zabielska
Beata Wójcik
Adrian Chabowski
Jan Górski
spellingShingle Piotr Zabielski
Agnieszka Urszula Błachnio-Zabielska
Beata Wójcik
Adrian Chabowski
Jan Górski
Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
PLoS ONE
author_facet Piotr Zabielski
Agnieszka Urszula Błachnio-Zabielska
Beata Wójcik
Adrian Chabowski
Jan Górski
author_sort Piotr Zabielski
title Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
title_short Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
title_full Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
title_fullStr Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
title_full_unstemmed Effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
title_sort effect of plasma free fatty acid supply on the rate of ceramide synthesis in different muscle types in the rat.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description Ceramide is a key compound in sphingolipid metabolism. Dynamics of ceramide synthesis is important in the several biological processes, such as induction of apoptosis or insulin resistance. So far, its de novo synthesis rate was evaluated indirectly, based on the content of the compound, its intermediates and the activity of respective enzymes. The aim of the present study was to directly measure ceramide synthesis rate (FSR) in different muscle types under varied plasma FFA supply in rat with the use of [U-13C] palmitate tracer and LC/MS/MS. The experiments were carried out on male Wistar rats, divided into three groups: 1-control, 2-with elevated plasma free fatty acid (FFA) concentration by means of intralipid and heparin, 3-with reduced plasma FFA concentration by means of nicotinic acid. The stable plasma FFA concentration and plasma [U-13C] palmitate enrichment was maintained for two hours by simultaneous infusion of the tracer and the respective compounds. At the end of the experiment, samples of blood from the abdominal aorta, the heart, diaphragm, soleus and white section of the gastrocnemius were taken. Muscle sphinganine, sphingosine and ceramide content and enrichment and plasma palmitate enrichment was measured with the use of LC/MS/MS. Plasma FFA concentration and composition was measured by means of gas-liquid chromatography. Under basal conditions ceramide FSR in the heart and the diaphragm was higher than in the soleus and the white gastrocnemius. Elevation in the plasma FFA concentration increased the FSR and ceramide content in each muscle, which correlated with increased HOMA-IR. The highest FSR was noted in the heart. Reduction in the plasma FFA concentration decreased ceramide FSR in each muscle type, which was accompanied by marked reduction in HOMA-IR. It is concluded that ceramide FSR depends on both the muscle type and the plasma FFA supply and is correlated with whole body insulin sensitivity under varying plasma FFA supply.
url http://europepmc.org/articles/PMC5667851?pdf=render
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