Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?

Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?

The stoichiometry of the Na+/Ca2+ exchanger (NCX) had been generally accepted as 3Na+:1 Ca2+. However, recently a challenging stoichiometry of 4:1 was proposed. Therefore, using guinea pig ventricular cells, we re-examined the stoichiometry by measuring the reversal potential of the NCX current and...

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Main Authors: Masamitsu Hinata, Junko Kimura
Format: Article
Language:English
Published: Elsevier 2004-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319323710
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spelling doaj-dbbb687e46f047459d53830f7e76697a2020-11-25T02:06:26ZengElsevierJournal of Pharmacological Sciences1347-86132004-01-019611518Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?Masamitsu Hinata0Junko Kimura1Department of Pharmacology, School of Medicine, Fukushima Medical University, Fukushima 960-1295, JapanDepartment of Pharmacology, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Japan; Corresponding author. FAX: +81-24-548-0575 E-mail: jkimura@fmu.ac.jpThe stoichiometry of the Na+/Ca2+ exchanger (NCX) had been generally accepted as 3Na+:1 Ca2+. However, recently a challenging stoichiometry of 4:1 was proposed. Therefore, using guinea pig ventricular cells, we re-examined the stoichiometry by measuring the reversal potential of the NCX current and intracellular Ca2+ concentrations under the whole-cell voltage clamp. We confirmed that the stoichiometry of NCX is 3:1 not 4:1. In addition, we explored the possible reasons for obtaining erroneous results of a 4:1 stoichiometry. Keywords:: Na+/Ca2+ exchanger (NCX), stoichiometry, cardiac myocytehttp://www.sciencedirect.com/science/article/pii/S1347861319323710
collection DOAJ
language English
format Article
sources DOAJ
author Masamitsu Hinata
Junko Kimura
spellingShingle Masamitsu Hinata
Junko Kimura
Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?
Journal of Pharmacological Sciences
author_facet Masamitsu Hinata
Junko Kimura
author_sort Masamitsu Hinata
title Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?
title_short Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?
title_full Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?
title_fullStr Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?
title_full_unstemmed Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?
title_sort forefront of na+/ca2+ exchanger studies: stoichiometry of cardiac na+/ca2+ exchanger; 3:1 or 4:1?
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2004-01-01
description The stoichiometry of the Na+/Ca2+ exchanger (NCX) had been generally accepted as 3Na+:1 Ca2+. However, recently a challenging stoichiometry of 4:1 was proposed. Therefore, using guinea pig ventricular cells, we re-examined the stoichiometry by measuring the reversal potential of the NCX current and intracellular Ca2+ concentrations under the whole-cell voltage clamp. We confirmed that the stoichiometry of NCX is 3:1 not 4:1. In addition, we explored the possible reasons for obtaining erroneous results of a 4:1 stoichiometry. Keywords:: Na+/Ca2+ exchanger (NCX), stoichiometry, cardiac myocyte
url http://www.sciencedirect.com/science/article/pii/S1347861319323710
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AT junkokimura forefrontofnaca2exchangerstudiesstoichiometryofcardiacnaca2exchanger31or41
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