The effect of interleukin-10 and transforming growth factor β-1 on HLA-DR expression in colonic epithelial cells

• Main Authors: M. J. Zimmerman, G. R. Radford-Smith, D. P. Jewell
• Format: Article
• Language: English
• Published: Hindawi Limited 1998-01-01
• Series: Mediators of Inflammation
• Subjects: InterLeukin-10; Transforming Growth Factor β1; γ-Interferon; HLA-DR; HT29 Cell Culture.
• Online Access: http://dx.doi.org/10.1080/09629359891315
• ISSN: 0962-9351; 1466-1861

The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor β-1 (TGF β1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with γ-interferon. The effects of IL-10 alone, TGF β1 alone, and IL-10 and TGF β1 in combination were studied. HLA-DR expression was assessed using flow cytometric analysis. γ-Interferon induced HLA-DR expression in a dose-dependent fashion. In the absence of γ-interferon, neither IL-10 nor TGF β1 induced HLA-DR expression. In isolation, neither IL10 nor TGF β1 downregulated HLA-DR expression. When IL-10 and TGF β1 were added in combination, small (6-30%) statistically significant reductions in HLA-DR expression were seen. The biological significance is unclear.