Protective effect of silver nano particles against ovarian polycystic induced by morphine in rat

• Main Authors: Elham Salami, Manizheh Karami, Ameneh Jafaryan Dehkordi, Mohammadreza Jalali Nadoushan, Abazar Hajnorouzi
• Format: Article
• Language: English
• Published: Iranian Society of Nanomedicine 2018-12-01
• Series: Nanomedicine Research Journal
• Subjects: polycystic ovary; morphine; intra-ventromedial hypothalamus; nanosilver particles; rat
• Online Access: http://www.nanomedicine-rj.com/article_33846_36cd6563bf07b6a2544ea6fe15a76172.pdf
• ISSN: 2476-3489; 2476-7123

Background and Objective: Morphine can cause harmful effects in the ovaries. The silver nanoparticles (Ag-NPs) used in health care because of antimicrobial properties, can diffuse into the brain blood barrier. This study investigated the protective effect of Ag-NPs on the induction of polycystic ovary (PCO) due to injection of morphine intra-ventromedial hypothalamus (intra-VMH) of rat compared with the animal receiving the drug (10-100 mg/kg) intraperitoneally (i.p.) twice daily for 7 days to lead to addiction. Materials and Methods: The rats (bought of Pasture Institute of Iran weighing 200-250 g) were housed under standard conditions and fed ad libitum. They were randomly divided to addict to drug or morphine (0.001 to 0.4 μg/rat) receiving into the VMH (AP: -1.92) a week after stereotaxic surgery. Ag-Nps (0.01, 0.001 and 0.0001 μg/rat) were administered intra-VMH alone or prior to morphine effective dose (0.4 μg/rat). Control group was given only saline. By the end of the treatment, the animals’ ovaries and/or brain were dissected and studied histopathologically. The ovaries were also checked by the marker of the NO, NADPH-diaphorase. Results: All experimental rats’ ovaries treated morphine showed polycystic characteristics and the NO activation was evidenced in the ovaries in the comparison with the saline group (p