Rat skin physiology is modified by age

Animal models have been useful in the study of specific mechanisms affecting human skin. Such is the case with ageing and the micromechanical changes determining wrinkles in UV- irradiated mice. These models enabled us to understand that ageing encompasses many peculiar mechanical responses which ca...

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Main Authors: Graziela Sponchiado, C Mello-Sampayo, Maria M Pereira, Henrique Silva, MF Otuki, B Silva-Lima, Luis Monteiro Rodrigues
Format: Article
Language:English
Published: ALIES - Associação Lusófona para o Desenvolvimento da Investigação e do Ensino das Ciências da Saúde 2012-12-01
Series:Journal Biomedical and Biopharmaceutical Research (BBR)
Subjects:
Online Access:http://www.alies.pt/BBR%20Editions/Vol-9-2-2012/Article8_9n2.pdf
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spelling doaj-db716fda4e704d539b6ccb6227d859882020-11-25T01:23:59ZengALIES - Associação Lusófona para o Desenvolvimento da Investigação e do Ensino das Ciências da SaúdeJournal Biomedical and Biopharmaceutical Research (BBR) 2182-23602182-23792012-12-019219920610.19277/bbr.9.2.42Rat skin physiology is modified by ageGraziela Sponchiado0C Mello-Sampayo1Maria M Pereira2Henrique Silva3MF Otuki4B Silva-Lima5Luis Monteiro Rodrigues6iMed Research Institute for Medicines and Pharmaceutical Sciences, Universidade de Lisboa – Fac Pharmacy; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.iMed Research Institute for Medicines and Pharmaceutical Sciences, Universidade de Lisboa – Fac Pharmacy; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.CBIOS – Universidade Lusófona's Research Center for Health Science and TechnologiesCBIOS – Universidade Lusófona's Research Center for Health Science and Technologies; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil; Department of Pharmaceutical Sciences, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, BraziliMed Research Institute for Medicines and Pharmaceutical Sciences, Universidade de Lisboa – Fac Pharmacy; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.CBIOS – Universidade Lusófona's Research Center for Health Science and Technologies; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.Animal models have been useful in the study of specific mechanisms affecting human skin. Such is the case with ageing and the micromechanical changes determining wrinkles in UV- irradiated mice. These models enabled us to understand that ageing encompasses many peculiar mechanical responses which cannot be explained by homogeneous deformation of the skin. Moreover, a major aspect to consider is that ageing processes differ among species and direct inferences are rarely possible. This project aimed to compare age-induced changes in skin properties by studying two Wistar rat groups of different ages – young adult rats (n=7, 20–24 week-old) and old adult rats (n=5, 48–72 week-old). Non-invasive measuring approaches involving transepidermal water loss (TEWL), superficial hydration (MoistureMeter), and biomechanics (Reviscometer, and Cutometer by MPA80) were taken in the animal dorsum (neck, bottom) over a period of five days, allowing statistical comparisons between groups. Results revealed no significant differences for TEWL, firmness or viscoelasticity between the two groups. However, a significant hydration decrease, of nearly 40%, was noted in the old adult rats group. These results, although preliminary, if translatable to humans could be useful to address age-related changes in processes such as healing, or patch dressing efficacy/safety where hydration is a critical determinant. Their potential use for the prediction of efficacy and or safety of topically applied products for the elderly as compared to young adults may warrant further investigation.. http://www.alies.pt/BBR%20Editions/Vol-9-2-2012/Article8_9n2.pdfWistar ratTEWLageinghydrationbiomechanics
collection DOAJ
language English
format Article
sources DOAJ
author Graziela Sponchiado
C Mello-Sampayo
Maria M Pereira
Henrique Silva
MF Otuki
B Silva-Lima
Luis Monteiro Rodrigues
spellingShingle Graziela Sponchiado
C Mello-Sampayo
Maria M Pereira
Henrique Silva
MF Otuki
B Silva-Lima
Luis Monteiro Rodrigues
Rat skin physiology is modified by age
Journal Biomedical and Biopharmaceutical Research (BBR)
Wistar rat
TEWL
ageing
hydration
biomechanics
author_facet Graziela Sponchiado
C Mello-Sampayo
Maria M Pereira
Henrique Silva
MF Otuki
B Silva-Lima
Luis Monteiro Rodrigues
author_sort Graziela Sponchiado
title Rat skin physiology is modified by age
title_short Rat skin physiology is modified by age
title_full Rat skin physiology is modified by age
title_fullStr Rat skin physiology is modified by age
title_full_unstemmed Rat skin physiology is modified by age
title_sort rat skin physiology is modified by age
publisher ALIES - Associação Lusófona para o Desenvolvimento da Investigação e do Ensino das Ciências da Saúde
series Journal Biomedical and Biopharmaceutical Research (BBR)
issn 2182-2360
2182-2379
publishDate 2012-12-01
description Animal models have been useful in the study of specific mechanisms affecting human skin. Such is the case with ageing and the micromechanical changes determining wrinkles in UV- irradiated mice. These models enabled us to understand that ageing encompasses many peculiar mechanical responses which cannot be explained by homogeneous deformation of the skin. Moreover, a major aspect to consider is that ageing processes differ among species and direct inferences are rarely possible. This project aimed to compare age-induced changes in skin properties by studying two Wistar rat groups of different ages – young adult rats (n=7, 20–24 week-old) and old adult rats (n=5, 48–72 week-old). Non-invasive measuring approaches involving transepidermal water loss (TEWL), superficial hydration (MoistureMeter), and biomechanics (Reviscometer, and Cutometer by MPA80) were taken in the animal dorsum (neck, bottom) over a period of five days, allowing statistical comparisons between groups. Results revealed no significant differences for TEWL, firmness or viscoelasticity between the two groups. However, a significant hydration decrease, of nearly 40%, was noted in the old adult rats group. These results, although preliminary, if translatable to humans could be useful to address age-related changes in processes such as healing, or patch dressing efficacy/safety where hydration is a critical determinant. Their potential use for the prediction of efficacy and or safety of topically applied products for the elderly as compared to young adults may warrant further investigation..
topic Wistar rat
TEWL
ageing
hydration
biomechanics
url http://www.alies.pt/BBR%20Editions/Vol-9-2-2012/Article8_9n2.pdf
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