Rat skin physiology is modified by age
Animal models have been useful in the study of specific mechanisms affecting human skin. Such is the case with ageing and the micromechanical changes determining wrinkles in UV- irradiated mice. These models enabled us to understand that ageing encompasses many peculiar mechanical responses which ca...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
ALIES - Associação Lusófona para o Desenvolvimento da Investigação e do Ensino das Ciências da Saúde
2012-12-01
|
Series: | Journal Biomedical and Biopharmaceutical Research (BBR) |
Subjects: | |
Online Access: | http://www.alies.pt/BBR%20Editions/Vol-9-2-2012/Article8_9n2.pdf |
id |
doaj-db716fda4e704d539b6ccb6227d85988 |
---|---|
record_format |
Article |
spelling |
doaj-db716fda4e704d539b6ccb6227d859882020-11-25T01:23:59ZengALIES - Associação Lusófona para o Desenvolvimento da Investigação e do Ensino das Ciências da SaúdeJournal Biomedical and Biopharmaceutical Research (BBR) 2182-23602182-23792012-12-019219920610.19277/bbr.9.2.42Rat skin physiology is modified by ageGraziela Sponchiado0C Mello-Sampayo1Maria M Pereira2Henrique Silva3MF Otuki4B Silva-Lima5Luis Monteiro Rodrigues6iMed Research Institute for Medicines and Pharmaceutical Sciences, Universidade de Lisboa – Fac Pharmacy; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.iMed Research Institute for Medicines and Pharmaceutical Sciences, Universidade de Lisboa – Fac Pharmacy; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.CBIOS – Universidade Lusófona's Research Center for Health Science and TechnologiesCBIOS – Universidade Lusófona's Research Center for Health Science and Technologies; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil; Department of Pharmaceutical Sciences, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, BraziliMed Research Institute for Medicines and Pharmaceutical Sciences, Universidade de Lisboa – Fac Pharmacy; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.CBIOS – Universidade Lusófona's Research Center for Health Science and Technologies; Pharm.Sc Department, Universidade de Lisboa – Fac Pharmacy, Lisboa, Portugal.Animal models have been useful in the study of specific mechanisms affecting human skin. Such is the case with ageing and the micromechanical changes determining wrinkles in UV- irradiated mice. These models enabled us to understand that ageing encompasses many peculiar mechanical responses which cannot be explained by homogeneous deformation of the skin. Moreover, a major aspect to consider is that ageing processes differ among species and direct inferences are rarely possible. This project aimed to compare age-induced changes in skin properties by studying two Wistar rat groups of different ages – young adult rats (n=7, 20–24 week-old) and old adult rats (n=5, 48–72 week-old). Non-invasive measuring approaches involving transepidermal water loss (TEWL), superficial hydration (MoistureMeter), and biomechanics (Reviscometer, and Cutometer by MPA80) were taken in the animal dorsum (neck, bottom) over a period of five days, allowing statistical comparisons between groups. Results revealed no significant differences for TEWL, firmness or viscoelasticity between the two groups. However, a significant hydration decrease, of nearly 40%, was noted in the old adult rats group. These results, although preliminary, if translatable to humans could be useful to address age-related changes in processes such as healing, or patch dressing efficacy/safety where hydration is a critical determinant. Their potential use for the prediction of efficacy and or safety of topically applied products for the elderly as compared to young adults may warrant further investigation.. http://www.alies.pt/BBR%20Editions/Vol-9-2-2012/Article8_9n2.pdfWistar ratTEWLageinghydrationbiomechanics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Graziela Sponchiado C Mello-Sampayo Maria M Pereira Henrique Silva MF Otuki B Silva-Lima Luis Monteiro Rodrigues |
spellingShingle |
Graziela Sponchiado C Mello-Sampayo Maria M Pereira Henrique Silva MF Otuki B Silva-Lima Luis Monteiro Rodrigues Rat skin physiology is modified by age Journal Biomedical and Biopharmaceutical Research (BBR) Wistar rat TEWL ageing hydration biomechanics |
author_facet |
Graziela Sponchiado C Mello-Sampayo Maria M Pereira Henrique Silva MF Otuki B Silva-Lima Luis Monteiro Rodrigues |
author_sort |
Graziela Sponchiado |
title |
Rat skin physiology is modified by age |
title_short |
Rat skin physiology is modified by age |
title_full |
Rat skin physiology is modified by age |
title_fullStr |
Rat skin physiology is modified by age |
title_full_unstemmed |
Rat skin physiology is modified by age |
title_sort |
rat skin physiology is modified by age |
publisher |
ALIES - Associação Lusófona para o Desenvolvimento da Investigação e do Ensino das Ciências da Saúde |
series |
Journal Biomedical and Biopharmaceutical Research (BBR) |
issn |
2182-2360 2182-2379 |
publishDate |
2012-12-01 |
description |
Animal models have been useful in the study of specific mechanisms affecting human skin. Such is the case with ageing and the micromechanical changes determining wrinkles in UV- irradiated mice. These models enabled us to understand that ageing encompasses many peculiar mechanical responses which cannot be explained by homogeneous deformation of the skin. Moreover, a major aspect to consider is that ageing processes differ among species and direct inferences are rarely possible. This project aimed to compare age-induced changes in skin properties by studying two Wistar rat groups of different ages – young adult rats (n=7, 20–24 week-old) and old adult rats (n=5, 48–72 week-old). Non-invasive measuring approaches involving transepidermal water loss (TEWL), superficial hydration (MoistureMeter), and biomechanics (Reviscometer, and Cutometer by MPA80) were taken in the animal dorsum (neck, bottom) over a period of five days, allowing statistical comparisons between groups. Results revealed no significant differences for TEWL, firmness or viscoelasticity between the two groups. However, a significant hydration decrease, of nearly 40%, was noted in the old adult rats group. These results, although preliminary, if translatable to humans could be useful to address age-related changes in processes such as healing, or patch dressing efficacy/safety where hydration is a critical determinant. Their potential use for the prediction of efficacy and or safety of topically applied products for the elderly as compared to young adults may warrant further investigation..
|
topic |
Wistar rat TEWL ageing hydration biomechanics |
url |
http://www.alies.pt/BBR%20Editions/Vol-9-2-2012/Article8_9n2.pdf |
work_keys_str_mv |
AT grazielasponchiado ratskinphysiologyismodifiedbyage AT cmellosampayo ratskinphysiologyismodifiedbyage AT mariampereira ratskinphysiologyismodifiedbyage AT henriquesilva ratskinphysiologyismodifiedbyage AT mfotuki ratskinphysiologyismodifiedbyage AT bsilvalima ratskinphysiologyismodifiedbyage AT luismonteirorodrigues ratskinphysiologyismodifiedbyage |
_version_ |
1725119671340892160 |