Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).

Abscisic acid (ABA) is a key component of the signaling system that integrates plant adaptive responses to abiotic stress. Overexpression of Arabidopsis molybdenum cofactor sulfurase gene (LOS5) in maize markedly enhanced the expression of ZmAO and aldehyde oxidase (AO) activity, leading to ABA accu...

Full description

Bibliographic Details
Main Authors: Yao Lu, Yajun Li, Jiachang Zhang, Yitao Xiao, Yuesen Yue, Liusheng Duan, Mingcai Zhang, Zhaohu Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3542365?pdf=render
id doaj-db627b5774bd474da9fdfc6af3fb57fa
record_format Article
spelling doaj-db627b5774bd474da9fdfc6af3fb57fa2020-11-24T21:35:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5212610.1371/journal.pone.0052126Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).Yao LuYajun LiJiachang ZhangYitao XiaoYuesen YueLiusheng DuanMingcai ZhangZhaohu LiAbscisic acid (ABA) is a key component of the signaling system that integrates plant adaptive responses to abiotic stress. Overexpression of Arabidopsis molybdenum cofactor sulfurase gene (LOS5) in maize markedly enhanced the expression of ZmAO and aldehyde oxidase (AO) activity, leading to ABA accumulation and increased drought tolerance. Transgenic maize (Zea mays L.) exhibited the expected reductions in stomatal aperture, which led to decreased water loss and maintenance of higher relative water content (RWC) and leaf water potential. Also, transgenic maize subjected to drought treatment exhibited lower leaf wilting, electrolyte leakage, malondialdehyde (MDA) and H(2)O(2) content, and higher activities of antioxidative enzymes and proline content compared to wild-type (WT) maize. Moreover, overexpression of LOS5 enhanced the expression of stress-regulated genes such as Rad 17, NCED1, CAT1, and ZmP5CS1 under drought stress conditions, and increased root system development and biomass yield after re-watering. The increased drought tolerance in transgenic plants was associated with ABA accumulation via activated AO and expression of stress-related gene via ABA induction, which sequentially induced a set of favorable stress-related physiological and biochemical responses.http://europepmc.org/articles/PMC3542365?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Yao Lu
Yajun Li
Jiachang Zhang
Yitao Xiao
Yuesen Yue
Liusheng Duan
Mingcai Zhang
Zhaohu Li
spellingShingle Yao Lu
Yajun Li
Jiachang Zhang
Yitao Xiao
Yuesen Yue
Liusheng Duan
Mingcai Zhang
Zhaohu Li
Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
PLoS ONE
author_facet Yao Lu
Yajun Li
Jiachang Zhang
Yitao Xiao
Yuesen Yue
Liusheng Duan
Mingcai Zhang
Zhaohu Li
author_sort Yao Lu
title Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
title_short Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
title_full Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
title_fullStr Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
title_full_unstemmed Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
title_sort overexpression of arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (zea mays l.).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Abscisic acid (ABA) is a key component of the signaling system that integrates plant adaptive responses to abiotic stress. Overexpression of Arabidopsis molybdenum cofactor sulfurase gene (LOS5) in maize markedly enhanced the expression of ZmAO and aldehyde oxidase (AO) activity, leading to ABA accumulation and increased drought tolerance. Transgenic maize (Zea mays L.) exhibited the expected reductions in stomatal aperture, which led to decreased water loss and maintenance of higher relative water content (RWC) and leaf water potential. Also, transgenic maize subjected to drought treatment exhibited lower leaf wilting, electrolyte leakage, malondialdehyde (MDA) and H(2)O(2) content, and higher activities of antioxidative enzymes and proline content compared to wild-type (WT) maize. Moreover, overexpression of LOS5 enhanced the expression of stress-regulated genes such as Rad 17, NCED1, CAT1, and ZmP5CS1 under drought stress conditions, and increased root system development and biomass yield after re-watering. The increased drought tolerance in transgenic plants was associated with ABA accumulation via activated AO and expression of stress-related gene via ABA induction, which sequentially induced a set of favorable stress-related physiological and biochemical responses.
url http://europepmc.org/articles/PMC3542365?pdf=render
work_keys_str_mv AT yaolu overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT yajunli overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT jiachangzhang overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT yitaoxiao overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT yuesenyue overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT liushengduan overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT mingcaizhang overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
AT zhaohuli overexpressionofarabidopsismolybdenumcofactorsulfurasegeneconfersdroughttoleranceinmaizezeamaysl
_version_ 1725944319111593984