| Summary: | Aqueous extract of Aconitum carmichaeli Debx is fermented by saccharomyces cerevisiae where the transformations in alkaloids are analyzed with HPLC. In the yeast-fermented Aconitum chloroform extract, the content of hypaconitine is lessened from 63.6% to 14.8%; the content of songorine increases from 7.5% to 34.5% in relation to original Aconitum. In tail clip test on mice, both original (ED50, 0.25 ± 0.04 mg/kg) and yeast- fermented Aconitum (ED50, 0.44 ± 0.12 mg/kg) showed a potent analgesic action. However, the fermentation process may certainly relieve the toxicity of Aconitum. The LD50 values of original and yeast- fermented Aconitum are about 1.44 mg/kg and 13.80 mg/kg, respectively, but the latter has a greater therapeutic scope than the former. This discovery may provide a way to develop new analgesics.
|
|---|
